| 货号 | 3203T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb detects endogenous levels of IRS-1 only when phosphorylated at Ser612. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10). |
| 存放说明 | -20C |
| 计算分子量 | 180 |
| 参考文献 | 1 . Sun, X.J. et al. (1991) Nature 352, 73-77. 2 . Sun, X.J. et al. (1992) J. Biol. Chem. 267, 22662-22672. 3 . Myers Jr., M.G. et al. (1993) Endocrinology 132, 1421-1430. 4 . Wang, L.M. et al. (1993) Science 261, 1591-1594. 5 . Rui, L. et al. (1997) J. Clin. Invest. 107, 181-189. 6 . Gao, Z. et al. (2002) J. Biol. Chem. 277, 48115-48121. 7 . Horike, N. et al. (2003) J. Biol. Chem. 278, 18440-18447. 8 . Ozes, O.N. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 4640-4645. 9 . De Fea, K. and Ruth, R.A. (1997) Biochemistry 36, 12939-12947. 10 . Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307. 11 . Andreozzi, F. et al. (2004) Endocrinology 145, 2845-57. |
Western blot analysis of cell extracts from CHO IR/IRS-1 cells, untreated or treated with insulin, using Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb (upper and middle) or IRS-1 Antibody #2382 (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing. Wetern blot检测CHO IR/IRS-1细胞提取物，细胞未处理或用胰岛素处理，所用抗体为Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb兔单抗 (上中)和IRS-1 Antibody #2382 (下)。中间道事先用小肠磷酸酶进行处理。 |
