| 货号 | 4191T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , IF-IC |
| 目标/特异性 | Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb detects endogenous levels of stathmin protein only when phosphorylated at Ser38. |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:8000) IF-IC (1:12800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Stathmin is a ubiquitously expressed microtubule destabilizing phosphoprotein that is upregulated in a number of cancers. The amino terminus of the protein contains multiple phosphorylation sites and is involved in the promotion of tubulin filament depolymerization. Phosphorylation at these sites inactivates the protein and stabilizes microtubules. Ser16 phosphorylation by CaM kinases II and IV (1,2) increases during G2/M-phase and is involved in mitotic spindle regulation (3,4). Ser38 is a target for cdc2 kinase (5) and TNF-induced cell death gives rise to reactive oxygen intermediates leading to hyperphosphorylation of stathmin (6). EGF receptor activation of Rac and cdc42 also increases phosphorylation of stathmin on Ser16 and Ser38 (7). Other closely related family members are neuronally expressed and include SCG10, SCLIP, RB3 and its splice variants RB3 and RB3. Stathmin and SCG10 have been shown to play roles in neuronal-like development in PC12 cells (8). |
| 存放说明 | -20C |
| 计算分子量 | 19, 20 |
| 参考文献 | 1 . Marklund, U. et al. (1994) Eur J Biochem 225, 53-60. 2 . le Gouvello, S. et al. (1998) J Immunol 161, 1113-22. 3 . Mistry, S.J. and Atweh, G.F. (2001) J Biol Chem 276, 31209-15. 4 . Gavet, O. et al. (1998) J Cell Sci 111 ( Pt 22), 3333-46. 5 . Luo, X.N. et al. (1994) J Biol Chem 269, 10312-8. 6 . Vancompernolle, K. et al. (2000) J Biol Chem 275, 33876-82. 7 . Daub, H. et al. (2001) J Biol Chem 276, 1677-80. 8 . Di Paolo, G. et al. (1996) J Cell Biol 133, 1383-90. |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or λ phosphatase-treated (right) using Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb. 使用Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb，免疫组化分析人源乳腺癌组织石蜡切片，组织分为control (左图)或λ phosphatase-treated (右图)。 | |
Immunohistochemical analysis of paraffin-embedded HT-29 cell pellets, untreated (left) or nocodazole-treated (right), using Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb. 使用Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb，免疫组化分析HT-29细胞石蜡切片，组织分为control (左图)或nocodazole-treated (右图)。 | |
Confocal immunofluorescent analysis of HT-29 cells, untreated (left) or λ phosphatase-treated (right), using Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb (绿色)标记，共聚焦免疫荧光分析HT-29细胞，细胞分为untreated (左图)和λ phosphatase-treated (right)。DY-554 phalloidin标记微丝蛋白(红色)。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。 | |
Western blot analysis of extracts from HT-29 cells, untreated or treated with nocodazole alone (100 ng/mL 24 hours) or nocodazole followed by λ and calf intestinal phosphatases, using Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb. 使用Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb，免疫印迹（Western Blot）分析HT-29细胞中Phospho-Stathmin蛋白水平。细胞分为untreated或nocodazole alone (100 ng/mL 24 hours)或nocodazole随后λ和calf intestinal phosphatases处理。 | |
Western blot analysis of extracts from HT-29 and U-2 OS cells, untreated or synchronized in mitosis, using Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb. Mitotic synchrony was performed by using either a thymidine block followed by release into 100 ng/mL nocodazole for 24 hours or using 100 ng/mL Docetaxel #9886 for 24 hours. 使用Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb，免疫印迹（Western Blot）分析HT-29和U-2 OS细胞中Phospho-Stathmin蛋白水平。细胞分为untreated或Mitotic synchrony。Mitotic synchrony通过使用thymidine封闭随后100 ng/mL nocodazole处理24小时或使用100 ng/mL Docetaxel #9886 处理24小时。 |
