| 货号 | 13008T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Phospho-YAP (Ser127) (D9W2I) Rabbit mAb recognizes endogenous levels of YAP protein only when phosphorylated at Ser127. |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:2500) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5,6). Upon phosphorylation at Ser127, YAP interacts with 14-3-3 in an Akt-dependent manner and suppresses p73-mediated apoptosis (6). |
| 存放说明 | -20C |
| 计算分子量 | 65-75 |
| 参考文献 | 1 . Sudol, M. (1994) Oncogene 9, 2145-52. 2 . Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90. 3 . Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23. 4 . Sudol, M. et al. (1995) FEBS Lett 369, 67-71. 5 . Yagi, R. et al. (1999) EMBO J 18, 2551-62. 6 . Basu, S. et al. (2003) Mol Cell 11, 11-23. 7 . Zhao, B. et al. (2010) Genes Dev 24, 862-74. 8 . Zhao, B. et al. (2007) Genes Dev 21, 2747-61. 9 . Yu, F.X. et al. (2012) Cell 150, 780-91. 10 . Zhao, B. et al. (2010) Genes Dev 24, 72-85. |
Western blot analysis of extracts from PANC-1 cells, untreated (-) or λ-phosphatase-treated (+), using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb (upper), YAP Antibody #4912 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). 未处理(-)或λ磷酸酶处理(-)的PANC-1细胞提取物，使用Phospho-YAP (Ser127) (D9W2I) Rabbit mAb兔单抗(上)，YAP Antibody #4912(下)和β-Actin (D6A8) Rabbit mAb #8457(下)进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma, control (left) or λ-phosphatase treated (right), using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb. 对照(左)或λ-磷酸酶处理(右)处理的石蜡包埋人结肠癌组织，使用Phospho-YAP (Ser127) (D9W2I) Rabbit mAb进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 在对照肽段(左)或抗原特异性肽段(右)存在的条件下，使用hospho-YAP (Ser127) (D9W2I) Rabbit mAb兔单抗对石蜡包埋的人非小细胞肺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded cell pellets, A-204 (left) and RL-7 (right), using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb. 使用Phospho-YAP (Ser127) (D9W2I) Rabbit mAb兔单抗对石蜡包埋的A-204细胞 (左) 和 RL-7 细胞(右)进行免疫组化分析。 | |
