Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb【科瑞奥博】

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Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb

货号： 4377T 基本售价： 1600.0 元规格： -

产品信息

概述
货号	4377T
同种亚型	Rabbit IgG
反应种属	Human,Mouse,Rat,Monkey,Mink,D. melanogaster,Zebrafish,Pig,
来源宿主	Rabbit IgG
应用	WB, F , IF-IC
目标/特异性	Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Tyr204. The antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase.
使用方法	WB(1:1000) F (1:200) IF-IC (1:200)

性能
供应商	CST
灵敏度	Endogenous
背景	Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.
存放说明	-20C
计算分子量	42, 44
参考文献	1 . Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44. 2 . Baccarini, M. (2005) FEBS Lett 579, 3271-7. 3 . Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39. 4 . Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310. 5 . Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74. 6 . Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75. 7 . Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505. 8 . Marais, R. et al. (1993) Cell 73, 381-93. 9 . Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24. 10 . Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.

参考图片

流式方法检测细胞提取物：U0126抑制的(蓝色)、PMA刺激的(绿色)Jurkat细胞，使用的抗体是Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb兔单抗，红色曲线表示采用非特异性阴性对照的结果。

Western blot analysis of purified MAPK phospho-proteins or extracts from NIH/3T3 cells treated with UV light and PDGF, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (upper), Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 (middle), and Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb #4671 (lower).

western blot方法检测细胞提取物：UV、PDGF处理的NIH/3T3细胞和纯化的MAPK磷酸化蛋白，使用的抗体是Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb 兔单抗(上图), Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215兔单抗 (中图)和Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb #4671兔单抗 (下图)。