| 货号 | 4406T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | Phospho-Jak2 (Tyr1007) (D15E2) Rabbit mAb detects endogenous levels of Jak2 when phosphorylated at Tyr1007. This antibody can also detect double phosphorylation at Tyr1007 and Tyr1008. Cross-reactivity may be observed with phosphorylated Jak3 and Tyk2. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. |
| 存放说明 | -20C |
| 计算分子量 | 125 |
| 参考文献 | 1 . Leonard, W.J. and OShea, J.J. (1998) Annu Rev Immunol 16, 293-322. 2 . Darnell, J.E. (1997) Science 277, 1630-5. 3 . VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93. 4 . Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92. 5 . Zhu, T. et al. (1998) J Biol Chem 273, 10682-9. |
Western blot analysis of extracts from UT-7 cells, untreated or treated with erythropoietin (EPO; 3 units/ml; 5 min) and from untreated HEL cells, using Phospho-Jak2 (Tyr1007) (D15E2) Rabbit mAb (upper) or total Jak2 (D2E12) Rabbit mAb #3230 (lower). HEL cells contain a V617F activating mutation in Jak2.Western免疫印迹。用Phospho-Jak2 (Tyr1007) (D15E2) Rabbit mAb (上图) 或 total Jak2 (D2E12) Rabbit mAb #3230 (下图) 研究经erythropoietin (EPO; 3 units/ml; 5 min)处理的UT-7细胞和未经处理的UT-7细胞及HEL细胞的细胞提取液。 |
