Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb【科瑞奥博】

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Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb

货号： 13969T 基本售价： 1600.0 元规格： 20ul

产品信息

概述
货号	13969T
同种亚型	Rabbit IgG
反应种属	Human/Mouse/Rat/Monkey
来源宿主	Rabbit IgG
应用	W/IP/IF-IC/ChIP
目标/特异性	Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb detects endogenous levels of histone H3 when tri-methylated on Lys9. This antibody shows some cross-reactivity with histone H3 that is di-methylated on Lys9, but does not cross-react with non-methylated or mono-methylated histone H3 Lys9. This antibody does not detect tri-methyl histone H3 Lys9 when the adjacent Ser10 residue is phosphorylated during mitosis. In addition, this antibody does not cross-react with methylated histone H3 Lys4, Lys27, Lys36, or Lys79.
使用方法	WB(1:1000) IP (1:50) F (1:50) IF-IC (1:800) ChIP (1:50)

性能
供应商	CST
灵敏度	Endogenous
背景
存放说明	-20C
计算分子量	17
参考文献	1 . Peterson, C.L. and Laniel, M.A. (2004) Curr Biol 14, R546-51. 2 . Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop , 1-27. 3 . Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42. 4 . Lee, D.Y. et al. (2005) Endocr Rev 26, 147-70. 5 . Daniel, J.A. et al. (2005) Cell Cycle 4, 919-26. 6 . Shi, X. et al. (2006) Nature 442, 96-9. 7 . Wysocka, J. et al. (2006) Nature 442, 86-90. 8 . Wysocka, J. et al. (2005) Cell 121, 859-72. 9 . Trojer, P. and Reinberg, D. (2006) Cell 125, 213-7.

参考图片

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ HeLa cells and either 10 μl of Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP^® Human GAPDH Exon 1 Primers #5516, SimpleChIP^® Human AFM Intron 1 Primers #5098, SimpleChIP^® Human MYT-1 Exon 1 Primers #4493, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Confocal immunofluorescent analysis of interphase (left) or mitotic (right) HeLa cells, untreated (upper) or λ phosphatase-treated (lower), using Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye). As shown, this antibody does not detect tri-methyl histone H3 Lys9 in mitotic cells when the adjacent Ser10 residue is phosphorylated.

Western blot analysis of extracts from various cell lines using Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb.

Antibody specificity was determined by western blotting. HeLa and NIH/3T3 cell lysates were probed with Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb (Panel A) or Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb pre-adsorbed with 1.5 μM of various competitor peptides (panels B-M). As shown, only the tri-methyl histone H3 (Lys9) peptide (panel E) competed away binding of the antibody.