Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb【科瑞奥博】

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Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb

货号： 5112T 基本售价： 1600.0 元规格： -

产品信息

概述
货号	5112T
同种亚型	Rabbit IgG
反应种属	Human,Mouse,Rat,Monkey,
来源宿主	Rabbit IgG
应用	WB, F
目标/特异性	Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb detects endogenous levels of ATF-2 only when phosphorylated at threonine 71. This antibody does not cross-react with phosphorylated c-Jun, CREB or other transcription factors. It recognizes both Thr69/Thr71 dually phosphorylated ATF-2 and Thr71 singly phosphorylated ATF-2 equally well.
使用方法	WB(1:1000) F (1:1600)

性能
供应商	CST
灵敏度	Endogenous
背景	The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).
存放说明	-20C
计算分子量	70
参考文献	1 . Abdel-Hafiz, H.A. et al. (1992) Mol Endocrinol 6, 2079-89. 2 . Gupta, S. et al. (1995) Science 267, 389-93. 3 . van Dam, H. et al. (1995) EMBO J 14, 1798-811. 4 . Livingstone, C. et al. (1995) EMBO J 14, 1785-97.

参考图片

Flow cytometric analysis of THP-1 cells, untreated (blue) or Anisomycin treated (green), using Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb compared to a nonspecific negative control antibody (red).Flow cytometric方法检测细胞提取物：未经处理（蓝色）和茴香霉素处理的（绿色）THP-1 细胞，使用的抗体是Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb，红色表示非特异性阴性对照抗体。

Western blot analysis of extracts from untreated or UV-treated COS cells, NIH/3T3 cells and C6 cells, using Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (upper), or ATF-2 (20F1) Rabbit mAb #9226 (lower).Western blot 方法检测细胞提取物：未经处理和紫外照射的COS、C6细胞，使用的抗体是Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (上图), or ATF-2 (20F1) Rabbit mAb #9226 （下图)。