Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb【科瑞奥博】

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Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb

货号： 3079T 基本售价： 1600.0 元规格： -

产品信息

概述
货号	3079T
同种亚型	Rabbit IgG
反应种属	Human,
来源宿主	Rabbit IgG
应用	WB, IF-IC
目标/特异性	Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb detects endogenous levels of Aurora A only when phosphorylated at Thr288.
使用方法	WB(1:1000) IF-IC (1:1600)

性能
供应商	CST
灵敏度	Endogenous
背景	Aurora A (AIK) is a cell cycle-regulated serine/threonine protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5) and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation and altered survivin localization (6).极光激酶A(AIK)是一种调节细胞周期的丝氨酸/苏氨酸蛋白激酶，在多种肿瘤细胞系中过表达(1-3)。在蛋白激酶激活回路中，极光激酶A苏氨酸(288位)的磷酸化可显著提高其活性并靶向有丝分裂过程中蛋白质的蛋白酶体降解(4)。与极光激酶A密切相关的极光激酶B(AIM1)在多重有丝分裂事件中发挥作用，siRNA沉默其表达之后可以导致组蛋白H3磷酸化减少、异常的染色体排列/分离以及生存蛋白的定位改变(6)。
存放说明	-20C
计算分子量	48
参考文献	1 . Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65. 2 . Zhou, H. et al. (1998) Nat Genet 20, 189-93. 3 . Sen, S. et al. (1997) Oncogene 14, 2195-200. 4 . Walter, A.O. et al. (2000) Oncogene 19, 4906-16. 5 . Kallio, M.J. et al. (2002) Curr Biol 12, 900-5. 6 . Hauf, S. et al. (2003) J Cell Biol 161, 281-94.

参考图片

Confocal immunofluorescent analysis of mitotic HeLa cells during metaphase (left) and anaphase (right) using Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb (red) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦荧光法检测有丝分裂期的HeLa细胞。左图为有丝分裂中期，右图为有丝分裂后期，使用的抗体为 Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb (红色) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (绿色)。蓝色伪彩为DNA荧光染料（产品信息为 DRAQ5®#4084 ）

Western blot analysis of extracts from hydroxyurea or nocodazole treated HeLa and HT29 cells using Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb.Western blot方法检测羟基脲或者诺考达唑处理的HeLa 和 HT29 细胞提取物，使用的抗体为Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb。