| 货号 | 2102T |
| 同种亚型 | Mouse IgG2b |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Mouse |
| 应用 | W/IP |
| 目标/特异性 | Non-phospho-Src (Tyr416) (7G9) Mouse mAb detects endogenous levels of Src only when dephosphorylated at tyrosine 416. The antibody cross-reacts with other Src family members (Lyn, Fyn, Lck, Yes and Hck) dephosphorylated at equivalent sites. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
| 参考文献 | 1 . Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609. 2 . Hunter, T. (1987) Cell 49, 1-4. |
Western blot analysis of extracts from pervanadate-treated (1 mM for 5 minutes) NIH/3T3 cells that have been stably transfected with a constitutively active form of Src (in which the regulatory tyrosine 527 residue has been mutated to phenylalanine), A431 cells and HepG2 cells, using Non-phospho-Src (Tyr416) (7G9 ) Mouse mAb (top), v-Src antibody (middle) or Phospho-Src (Tyr416) Antibody #2101 (bottom). |
