| 货号 | 8084T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Phospho-AMPA Receptor 1 (GluA1) (Ser845) (D10G5) Rabbit mAb recognizes endogenous levels of AMPA Receptor 1 (GluA1) protein only when phosphorylated at Ser845. While the literature refers to this residue as Ser845, it is Ser863 in the UniProt sequence P42261. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainite- and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the CNS. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). AMPARs that lack GluR 2 are permeable to calcium, in contrast to GluR 2 containing AMPARs (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Activity changes of AMPARs are implicated in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
| 参考文献 | 1 . Palmer, C.L. et al. (2005) Pharmacol Rev 57, 253-77. 2 . Cull-Candy, S. et al. (2006) Curr Opin Neurobiol 16, 288-97. 3 . Roche, K.W. et al. (1996) Neuron 16, 1179-88. 4 . Snyder, G.L. et al. (2000) J Neurosci 20, 4480-8. 5 . Lee, H.K. et al. (2000) Nature 405, 955-9. 6 . Lee, H.K. et al. (2003) Cell 112, 631-43. 7 . He, K. et al. (2009) Proc Natl Acad Sci U S A 106, 20033-8. 8 . Joiner, M.L. et al. (2010) EMBO J 29, 482-95. |
Western blot analysis of extracts from mouse brain and rat brain using Phospho-AMPA Receptor (GluR 1) (Ser845) (D10G5) Rabbit mAb. The phospho-specificity of the antibody was verified by blocking with a phospho or nonphosphopeptide. |
