| 货号 | 8556T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Phospho-FAK (Tyr397) (D20B1) Rabbit mAb detects FAK protein only when phosphorylated at Tyr397. This antibody may cross-react with overexpressed tyrosine phosphorylated protein such as EGFR. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 125 |
| 参考文献 | 1 . Parsons, J.T. et al. (2000) Oncogene 19, 5606-13. 2 . Schaller, M.D. et al. (1994) Mol Cell Biol 14, 1680-8. 3 . Cobb, B.S. et al. (1994) Mol Cell Biol 14, 147-55. 4 . Chen, H.C. et al. (1996) J Biol Chem 271, 26329-34. 5 . Zhang, X. et al. (1999) Proc Natl Acad Sci U S A 96, 9021-6. 6 . Calalb, M.B. et al. (1995) Mol Cell Biol 15, 954-63. 7 . Schlaepfer, D.D. et al. (1994) Nature 372, 786-791. |
Western blot analysis of extracts from A549 cells, untreated or treated with calf intestinal phosphatase (CIP), using Phospho-FAK (Tyr397) (D20B1) Rabbit mAb (upper) and FAK Antibody #3285 (lower).未处理或经过牛小肠碱性磷酸酶（CIP）处理的A549细胞提取物使用Phospho-FAK (Tyr397) (D20B1)兔单抗（上）和FAK抗体#3285（下）进行western blot分析。 |
