Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb【科瑞奥博】

产品中心

当前位置：首页>产品中心

Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb

货号： 14202T 基本售价： 1600.0 元规格： -

产品信息

概述
货号	14202T
同种亚型	Rabbit IgG
反应种属	Human,Mouse,Rat,
来源宿主	Rabbit IgG
应用	WB, IP
目标/特异性	Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb recognizes endogenous levels of ULK1 protein only when phosphorylated at Ser757 of mouse ULK1 (equivalent to Ser758 of human ULK1).
使用方法	WB(1:1000) IP (1:100)

性能
供应商	CST
灵敏度	Endogenous
背景	AMPK, activated during low nutrient conditions, directly phosphorylates ULK1 at multiple sites including Ser317, Ser555, and Ser777 (17,18). Conversely, mTOR, which is a regulator of cell growth and is an inhibitor of autophagy, phosphorylates ULK1 at Ser757 and disrupts the interaction between ULK1 and AMPK (17).
存放说明	-20C
计算分子量	140-150
参考文献	1 . Ogura, K. et al. (1994) Genes Dev 8, 2389-400. 2 . Kuroyanagi, H. et al. (1998) Genomics 51, 76-85. 3 . Yan, J. et al. (1998) Biochem Biophys Res Commun 246, 222-7. 4 . Yan, J. et al. (1999) Oncogene 18, 5850-9. 5 . Zhou, X. et al. (2007) Proc Natl Acad Sci USA 104, 5842-7. 6 . Tomoda, T. et al. (2004) Genes Dev 18, 541-58. 7 . Matsuura, A. et al. (1997) Gene 192, 245-50. 8 . Chan, E.Y. et al. (2007) J Biol Chem 282, 25464-74. 9 . Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. 10 . Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18. 11 . Stephan, J.S. and Herman, P.K. (2006) Autophagy 2, 146-8. 12 . Okazaki, N. et al. (2000) Brain Res Mol Brain Res 85, 1-12. 13 . Young, A.R. et al. (2006) J Cell Sci 119, 3888-900. 14 . Kamada, Y. et al. (2000) J Cell Biol 150, 1507-13. 15 . Lee, S.B. et al. (2007) EMBO Rep 8, 360-5. 16 . Hara, T. et al. (2008) J Cell Biol 181, 497-510. 17 . Kim, J. et al. (2011) Nat Cell Biol 13, 132-41. 18 . Egan, D.F. et al. (2011) Science 331, 456-61.

参考图片

Western blot analysis of extract from A172 cells, untreated (-) or treated with mTOR inhibitors, either Torin-1 (250 nM, 5 hrs), Torin-2 (250 nM, 5 hrs), or INK128 (250 nM, 5 hours) using Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb (upper) or ULK1 (D8H5) Rabbit mAb #8054 (lower).

Western blot analysis of extracts from various cell lines using Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb.