| 货号 | 8869T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Phospho-c-Cbl (Tyr700) (D16D7) Rabbit mAb recognizes endogenous levels of c-Cbl protein only when phosphorylated at Tyr700. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The c-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in hematopoietic cells (1,2). c-Cbl is rapidly tyrosine-phosphorylated in response to stimulation of a variety of cell-surface receptors and becomes associated with a number of intracellular signaling molecules such as protein tyrosine kinases, phosphatidylinositol-3 kinase, Crk, and 14-3-3 proteins (3,4). c-Cbl possesses a highly conserved amino-terminal phosphotyrosine binding domain (TKB) and a C3HC4 RING finger motif. The TKB recognizes phosphorylated tyrosines on activated receptor tyrosine kinases (RTKs) as well as other nonreceptor tyrosine kinases. The RING finger motif recruits ubiquitin-conjugating enzymes. These two domains are primarily responsible for the ubiquitin ligase activity of c-Cbl and downregulation of RTKs (3). Research studies have indicated that in human cancer tissues, c-Cbl is frequently tyrosine-phosphorylated in a tumor-specific manner (5). Phosphorylation of Tyr731 of c-Cbl provides a docking site for downstream signaling components such as p85 and Fyn (6). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
| 参考文献 | 1 . Blake, T.J. et al. (1991) Oncogene 6, 653-657. 2 . Thien, C.B. and Langdon, W.Y. (1998) Immunol. Cell Biol. 76, 473-482. 3 . Christine, B.F. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 294-307. 4 . Feshchenko, E.A. et al. (1998) J. Biol. Chem. 273, 8323-8331. 5 . Kamei, T. et al. (2000) Int. J. Oncol. 17, 335-339. 6 . Hunter, C. et al. (1999) J. Biol. Chem. 274, 2097-2106. 7 . Miura-Shimura, Y. et al. (2003) J Biol Chem 278, 38495-504. |
Immunoprecipitation (IP) of phosphorylated c-Cbl from Jurkat cell extracts, untreated (-) or treated with pervanadate (1 mM; +), using Phospho-c-Cbl (Tyr700) (D16D7) Rabbit mAb (lanes 4 and 6). Western blot detection was performed using the same antibody. Lanes 1 and 2 are 10% input. Lanes 3 and 5 are IPs performed with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900. 免疫共沉淀检测Jurkat细胞提取物中经过pervanadate (1 mM; +)处理或未经pervanadate处理的磷酸化的c-Cbl，采用Phospho-c-Cbl (Tyr700) (D16D7) Rabbit mAb (lanes 4 and 6)。Western blot检测采用相同的抗体。Lanes 1和2样品浓度为10%。Lanes 3和5 为免疫共沉淀采用Rabbit (DA1E) mAb IgG XP® 同型对照#3900。 | |
Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with pervanadate (1 mM; +), using Phospho-c-Cbl (Tyr700) (D16D7) Rabbit mAb (left). The phospho-specificity of the antibody was verified by preincubating the antibody with c-Cbl (Tyr700) phosphopeptide (center) or with c-Cbl (Tyr700) nonphosphopeptide prior to incubation with the membrane (right). Western blot检测经pervanadate (1 mM; +)处理或未经pervanadate处理的Jurkat细胞，采用Phospho-c-Cbl (Tyr700) (D16D7) Rabbit mAb (left)。在与细胞膜作用前，抗体预先与c-Cbl (Tyr700)磷酸肽(center)或c-Cbl (Tyr700)非磷酸肽孵育，表明抗体具有磷酸特异性。 |
