| 货号 | 9154T |
| 同种亚型 | Rabbit |
| 反应种属 | Human,Mouse,Rat,Monkey,D. melanogaster, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb detects endogenous levels of MEK1/2 only when activated by phosphorylation at Ser217/221. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII. |
| 存放说明 | -20C |
| 计算分子量 | 45 |
| 参考文献 | 1 . Crews, C.M. et al. (1992) Science 258, 478-480. 2 . Alessi, D.R. et al. (1994) EMBO J. 13, 1610-19. 3 . Rosen, L.B. et al. (1994) Neuron 12, 1207-21. 4 . Cowley, S. et al. (1994) Cell 77, 841-52. |
Western blot analysis of extracts from untreated or TPA-treated HeLa and NIH/3T3 cells, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (upper), or MEK1/2 Antibody #9122 (lower). |
