| 货号 | 9316T |
| 同种亚型 | Rabbit |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB, IHC-P |
| 目标/特异性 | Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb detects endogenous levels of GSK-3α when phosphorylated at Ser21 and does not detect GSK-3β when phosphorylated at Ser9. |
| 使用方法 | WB(1:1000) IHC-P (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). |
| 存放说明 | -20C |
| 计算分子量 | 51 |
| 参考文献 | 1 . Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9. 2 . Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41. 3 . Cross, D.A. et al. (1995) Nature 378, 785-9. 4 . Nusse, R. (1997) Cell 89, 321-3. 5 . Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511. |
Western blot 分析COS-7 细胞， λ磷酸酶和PDGF处理组，所用抗体为Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb兔单抗 (上) 或 GSK-3α Antibody #9338（下）。 | |
Western blot analysis of extracts from COS-7 cells, λ-phosphatase or PDGF-treated, using Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb (upper) or GSK-3α Antibody #9338 (lower). | |
