| 货号 | 9323T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IHC-P , IF-IC |
| 目标/特异性 | Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb detects endogenous levels of GSK-3β only when phosphorylated at Ser9. The antibody may cross-react weakly with the phosphorylated form of GSK-3α due to high sequence homology. |
| 使用方法 | WB(1:1000) IHC-P (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). |
| 存放说明 | -20C |
| 计算分子量 | 46 |
| 参考文献 | 1 . Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9. 2 . Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41. 3 . Cross, D.A. et al. (1995) Nature 378, 785-9. 4 . Nusse, R. (1997) Cell 89, 321-3. 5 . Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511. |
Western blot analysis of extracts from wild type (lanes 1,2), GSK-3α (-/-) (lanes 3,4) and GSK-3β (-/-) (lanes 5,6) mouse embryonic fibroblast cells (MEF), untreated or PDGF treated, using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb (upper) and GSK-3α/β Antibody (lower). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada). Western blot分析野生型（通道1和2），GSK-3α (-/-) (通道 3和4) 与 GSK-3β (-/-) (通道5和6) 的小鼠胚胎成纤维细胞，并分为未处理和PDGF处理组，使用的抗体为Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb兔单抗 (上) 和 GSK-3α/β Antibody (下)。（MEF 野生型, GSK-3α (-/-) 和 GSK-3β (-/-) 细胞是由加拿大多伦多大学的Jim Woodgett博士友情提供。） | |
Confocal immunofluorescent analysis of wild type (left), GSK-3α (-/-) (center), or GSK-3β (-/-) (right) mouse embryonic fibroblasts (MEFs), FGF-treated (top) or phosphatase-treated (bottom), using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada). 免疫共聚焦分析分析野生型（左），GSK-3α (-/-) (中) 与 GSK-3β (-/-) (右) 的小鼠胚胎成纤维细胞（MEFs），并分为FGF 处理（上）和phosphatase处理组（下），使用的抗体为Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb (绿色)。肌动蛋白纤维被DY-554环肽所染色（红色）。Blue pseudocolor为 DRAQ5® #4084 （ DNA染料的荧光)。（MEF 野生型, GSK-3α (-/-) 和 GSK-3β (-/-) 细胞由加拿大多伦多大学的Jim Woodgett博士友情提供。） | |
Immunohistochemical analysis using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb on SignalSlide(TM) Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right)). 免疫组化分析SignalSlide(TM) Phospho-Akt (Ser473) IHC 对照#8101（分别为未处理（左）或者 LY294002处理的石蜡包埋的LNCaP细胞），使用的抗体是Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded wild type (left) or GSK-3β (-/-) MEFs, using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb. (MEF wild type, GSK-3α knock out and GSK-3β knock out cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).免疫组化分析石蜡包埋的野生型或者GSK-3β (-/-) MEFs细胞，所用抗体为Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb。)。（MEF 野生型, GSK-3α (-/-) 和 GSK-3β (-/-) 细胞是由加拿大多伦多大学的Jim Woodgett博士友情提供。） | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-GSK3β (Ser9) (5B3) Rabbit mAb. 免疫组化分析石蜡包埋的人乳腺癌细胞，所用抗体为Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from NIH/3T3 cells, λ-phosphatase- or PDGF-treated, using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb (upper) or GSK-3β (27C10) Rabbit mAb #9315 (lower). Western blot 分析NIH/3T3 细胞， λ磷酸酶和PDGF处理组，所用抗体为Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb兔单抗 (上) 或GSK-3β (27C10) Rabbit mAb兔单抗 #9315 (下)。 |
