| 货号 | 9549T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb detects endogenous levels of PTEN only when phosphorylated at Ser380, Thr382 and Thr383. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10). |
| 存放说明 | -20C |
| 计算分子量 | 54 |
| 参考文献 | 1 . Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5. 2 . Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7. 3 . Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8. 4 . Wan X and Helman LJ (2003) Oncogene 22, 8205–11 5 . Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91. 6 . Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8. 7 . Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8. 8 . Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30. 9 . Funamoto, S. et al. (2002) Cell 109, 611-23. 10 . Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610. |
Western blot analysis of extracts from HeLa and NIH/3T3 cells, using Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb. Membranes were either left untreated (-) or treated with (+) calf intestinal phosphatase (CIP) post Western transfer to verify phospho-specificity of the antibody. Western blot 分析HeLa 和NIH/3T3细胞的提取物，所用抗体为Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb兔单抗。左图为western 转膜后，膜的未处理组和采用牛肠磷酸酶(CIP)处理组，用来证实抗体的磷酸特异性。 |
