| 货号 | 9649T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey,Zebrafish,C. elegans, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , F , IF-IC , ChIP |
| 目标/特异性 | Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb detects endogenous levels of histone H3 only when acetylated on Lys9. This antibody does not cross-react with other acetylated histones. |
| 使用方法 | WB(1:1000) IP (1:25) IHC-P (1:800) F (1:200) IF-IC (1:400) ChIP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11). |
| 存放说明 | -20C |
| 计算分子量 | 17 |
| 参考文献 | 1 . Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79. 2 . Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41. 3 . Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5. 4 . Cheung, P. et al. (2000) Cell 103, 263-71. 5 . Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73. 6 . Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9. 7 . Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13. 8 . Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60. 9 . Goto, H. et al. (1999) J Biol Chem 274, 25543-9. 10 . Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85. 11 . Dai J et al. (2005) Genes Dev 19, 472–88 |
Immunohistochemical analysis of paraffin-embedded human gastic carcinoma using Acetyl-Histone H3 (K9) Rabbit mAb in the presence of non-acetyl-peptide (left) or K9 acetyl-peptide (right). 使用Acetyl-Histone H3(K9) Rabbit mAb兔单抗，免疫组化分析人胃癌组织石蜡切片，左图是非乙酰化的肽段，右图是K9乙酰化的肽段。 | |
Flow cytometric analysis of untreated HeLa cells using Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb #9649 versus propidium iodide (DNA content). Note positive staining in cycling cells (box). 与propidium iodide (DNA含量)比较，使用Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb #9649兔单抗，流式细胞仪分析未处理的HeLa细胞。显示在细胞周期中呈阳性染色 (方框)。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 10 μl of Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. 使用SimpleChIP®Enzymatic Chromatin IP Kit (Magnetic Beads) #9003，用4 x 106 HeLa细胞的交联染色质以及10 µl Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb或2 µl Normal Rabbit IgG #2729进行染色质免疫沉淀实验。使用SimpleChIP® Human RPL30 Exon 3 Primers #7014、SimpleChIP® Human GAPDH Exon 1 Primers #5516、SimpleChIP® Human MyoD1 Exon 1 Primers #4490和SimpleChIP® Human α Satellite Repeat Primers #4486，浓缩的DNA通过real-time PCR定量。在每个样品中免疫沉淀DNA的数量被当做一个相对于总input chromatin的数量的信号，这相当于一。 | |
Western blot analysis of lysates from HeLa and NIH/3T3 cells, untreated or TSA-treated (400 nM for 18 hours) using Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb. 使用Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb兔单抗，免疫印迹(Western blot)分析HeLa和NIH/3T3细胞中Acetyl-Histone H3 (Lys9)的蛋白水平，细胞分为untreated或TSA-treated (400 nM for 18 hours)。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with TSA #9950 (right), using Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). 使用Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb兔单抗 (绿色)，共聚焦免疫荧光分析HeLa细胞，细胞分为untreated (左图)和TSA-treated (#9950;右图)。DY-554 phalloidin标记微丝蛋白(红色)。 |
