| 货号 | 2511T |
| 同种亚型 | Mouse IgG2b |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P |
| 目标/特异性 | Phospho-Estrogen Receptor α (Ser118) (16J4) Mouse mAb detects endogenous levels of estrogen receptor α only when phosphorylated at serine 118. It does not cross-react with phosphorylated estrogen receptor β. |
| 使用方法 | WB(1:1000) IHC-P (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Estrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD) (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation provides an important mechanism to regulate ERα activity (3,4). ERα is phosphorylated on multiple sites (5). Ser104, 106, 118 and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serines plays an important role in regulating ERα activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). Phosphorylation of Ser167 may confer tamoxifen resistance in breast cancer patients (4).雌激素受体α(ERα)是类固醇受体超家族的一员，含有高度保守的DNA结合域（DBD）和配体结合域（LBD）（1）。ERα通过其雌激素非依赖性和雌激素依赖性激活域（分别为AF-1和AF-2），调控转录招募共激活因子蛋白，并与一般的转录机制相互作用（2）。磷酸化提供了调控ERα活性的重要机制（3,4）。 ERα有多个磷酸化位点（5）。丝氨酸（104，106，118和167位）都位于氨基末端的转录激活功能域AF-1，这些丝氨酸的磷酸化在调节ERα活性中起着重要作用。丝氨酸（118位）可能是转录调节激酶CDK7的底物（5）。丝氨酸（167位）可以被p90RSK和Akt磷酸化（4,6）。丝氨酸（167位）的磷酸化可能会使乳腺癌患者对他莫昔芬耐药（4）。 |
| 存放说明 | -20C |
| 计算分子量 | 66 |
| 参考文献 | 1 . Mangelsdorf, D.J. et al. (1995) Cell 83, 835-9. 2 . Glass, C.K. and Rosenfeld, M.G. (2000) Genes Dev 14, 121-41. 3 . Chen, D. et al. (1999) Mol Cell Biol 19, 1002-15. 4 . Campbell, R.A. et al. (2001) J Biol Chem 276, 9817-24. 5 . Chen, D. et al. (2000) Mol Cell 6, 127-37. 6 . Joel, P.B. et al. (1998) Mol Cell Biol 18, 1978-84. |
Western blot analysis of extracts from untransfected MCF-7 cells, and COS-1 cells transfected with wild-type or mutant ER alpha, stimulated with EGF and E2, using Phospho-Estrogen Receptor alpha (Ser118) (16J4) Mouse mAb (upper) or control estrogen receptor alpha antibody #2512 (lower).Western blot方法检测未转染的MCF-7细胞提取物和用野生型或雌激素受体α突变体转染、EGF和E2刺激的COS-1细胞提取物，使用的抗体为Phospho-Estrogen Receptor alpha (Ser118) (16J4) Mouse mAb (上图)或control estrogen receptor alpha antibody #2512（下图）。 | |
Immunohistochemical staining of phosphorylated estrogen receptor alpha in paraffin-embedded human breast carcinoma showing nuclear localization, using Phospho-Estrogen Receptor alpha (Ser118) (16J4) Mouse mAb.免疫组织化学方法对石蜡包埋的人乳腺癌组织的磷酸化雌激素受体α进行染色，显示细胞核位置，使用的抗体为Phospho-Estrogen Receptor alpha (Ser118) (16J4) Mouse mAb. |
