| 货号 | 2361T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | Phospho-c-Jun (Ser63) (54B3) Rabbit mAb detects endogenous levels of c-Jun only when phosphorylated at serine 63. |
| 使用方法 | WB(1:1000) IHC-P (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12). |
| 存放说明 | -20C |
| 计算分子量 | 48 |
| 参考文献 | 1 . Jochum, W. et al. (2001) Oncogene 20, 2401-12. 2 . Davis, R.J. (2000) Cell 103, 239-52. 3 . Hilberg, F. et al. (1993) Nature 365, 179-81. 4 . Raivich, G. et al. (2004) Neuron 43, 57-67. 5 . Behrens, A. et al. (2002) EMBO J 21, 1782-90. 6 . Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700. 7 . Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62. 8 . Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6. 9 . Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3. 10 . Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20. 11 . Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81. 12 . Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4. |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear localization, using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人肺癌组织，显示细胞核位置。使用的抗体为Phospho-c-Jun (Ser63) (54B3) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human skin (normal adjacent to hemangioma), using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人皮肤组织（一般毗邻血管瘤），使用的抗体为Phospho-c-Jun (Ser63) (54B3) Rabbit mAb。 | |
Western blot analysis of extracts from untreated or anisomycin-treated C6 cells, or untreated or UV-treated 293 cells, using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb (upper) or c-Jun (60A8) Rabbit mAb #9165 (lower).Western blot方法检测细胞提取物：未经处理的、茴香霉素处理的C6细胞，未经处理的、紫外照射的293细胞，使用的抗体是Phospho-c-Jun (Ser63) (54B3) Rabbit mAb (上图) 和c-Jun (60A8) Rabbit mAb #9165 (下图)。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma untreated (left) or lambda phosphatase-treated (right), using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人乳腺癌组织，左侧是对照组，右侧是lambda磷酸酶处理后的组织。使用的抗体为Phospho-c-Jun (Ser63) (54B3) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb in the presence of control peptide (left) or Phospho-c-Jun (Ser63) II Blocking Peptide (#1020) (right).免疫组织化学方法检测石蜡包埋的人乳腺癌组织，使用的抗体为Phospho-c-Jun (Ser63) (54B3) Rabbit mAb。左侧是对照组多肽，右侧是Phospho-c-Jun (Ser63) II Blocking Peptide (#1020)。 | |
Immunohistochemical analysis of paraffin-embedded NIH/3T3 cell pellets, control (left) or anisomycin-treated (right), using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb.免疫组织化学方法检测石蜡包埋的NIH/3T3细胞团，左侧为对照组，右侧为茴香霉素处理的。使用的抗体为Phospho-c-Jun (Ser63) (54B3) Rabbit mAb。 |
