| 货号 | 2243T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IHC-F |
| 目标/特异性 | Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb detects endogenous levels of ErbB2 only when phosphorylated at tyrosines 1221/1222. The antibody does not detect other activated Erb family members or other tyrosine-phosphorylated proteins. |
| 使用方法 | WB(1:1000) IHC-P (1:320) IHC-F (1:320) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5). |
| 存放说明 | -20C |
| 计算分子量 | 185 |
| 参考文献 | 1 . Muthuswamy, S.K. et al. (1999) Mol Cell Biol 19, 6845-57. 2 . Qian, X. et al. (1994) Proc Natl Acad Sci USA 91, 1500-4. 3 . Dittadi, R. and Gion, M. (2000) J Natl Cancer Inst 92, 1443-4. 4 . Klapper, L.N. et al. (2000) Cancer Res 60, 3384-8. 5 . Kwon, Y.K. et al. (1997) J Neurosci 17, 8293-9. |
Western blot analysis of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower). Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb specifically detects phosphorylated HER2/ErbB2 but not other phosphorylated tyrosine kinases.Western blot法检测表达不同激活酪氨酸激酶的细胞的提取物，使用抗体为：Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb (上) 或 Phospho-Tyrosine mAb (P-Tyr-100) #9411 (下)。其中抗体Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb 特意识别磷酸化的HER2/ErbB2 但不识别其它磷酸化的激酶。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing membrane localization, using Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb.免疫组化法检测石蜡包埋的人乳腺癌组织，可见胞膜着色。使用抗体为：Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human renal adenocarcinoma, using Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb in the presence of control peptide (left) or Phospho-HER2/ErbB2 (Tyr1221/1222) Blocking Peptide #1254 (right).免疫组化法检测石蜡包埋的人肾腺癌组织：左图，使用对照多肽；右图，使用Phospho-HER2/ErbB2 (Tyr1221/1222) Blocking Peptide #1254 。使用抗体为：Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human NCI-H358 xenograft, using Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb.免疫组化法检测石蜡包埋的人NCI-H358细胞的异种移植物，使用抗体为：Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded SkBr3 cell pellets untreated (left) EGF-treated (right) either untreated (top) or lambda-phosphatase-treated (bottom), using Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb.免疫组化法检测石蜡包埋的SkBr3细胞沉淀：左图，未处理；右图，EGF处理同时未经（上）或经过（下）lambda-phosphatase处理。使用抗体为：Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb。 | |
Immunohistochemical analysis of frozen HCC827 xenograft, using Phospho-Her2/ErbB2 (Tyr1221/1222)(6B12) Rabbit mAb.免疫组化法检测HCC827异种移植物的冰冻组织，使用抗体为：Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb。 |
