| 货号 | 17819T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat |
| 应用 | WB,IP,IHC,IF |
| 目标/特异性 | Calponin 1 (D8L2T) Rabbit mAb recognizes endogenous levels of total Calponin 1 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:200) Immunohistochemistry (Paraffin) (1:1000) Immunofluorescence (Frozen) (1:100) Immunofluorescence (Immunocytochemistry) (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Calponin 1 is a member of the Calponin family, which consists of Calponins 1, 2, and 3. Calponin 1 is exquisitely expressed in smooth muscle cells of various tissues, where it interacts with filamentous F-actin to regulate smooth muscle contraction (1). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
| 参考文献 | 1 . Liu, R. and Jin, J.P. (2016) Gene 585, 143-53. |
Immunohistochemical analysis of paraffin-embedded human clear cell adenocarcinoma of the ovary using Calponin 1 (D8L2T) XP® Rabbit mAb. | |
Western blot analysis of extracts from SNB75, C2C12, and 293T cells using Calponin 1 (D8L2T) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). 293T cells are negative as expected. | |
Immunoprecipitation of Calponin 1 from SNB75 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP® Isotype Control #3900, and lane 3 is Calponin 1 (D8L2T) XP®Rabbit mAb. Western blot analysis was performed using Calponin 1 (D8L2T) XP®Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded SNB75 cell pellet (left, positive) and MDA-MB-231 cell pellet (right, negative) using Calponin 1 (D8L2T) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human invasive adenocarcinoma of the colon using Calponin 1 (D8L2T) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Calponin 1 (D8L2T) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of mouse colon (left) and skeletal muscle (right) using Calponin 1 (D8L2T) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of SNB75 cells (left, positive) and MCF10A cells (right, negative) using Calponin 1 (D8L2T) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
