| 货号 | 99966T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H |
| 来源宿主 | Rabbit IgG |
| 应用 | W IHC-P |
| 目标/特异性 | Mesothelin (D9R5G) XP® Rabbit mAb recognizes endogenous levels of total mesothelin protein. The antibody detects both uncleaved (full-length) and cleaved forms of mesothelin, but does not detect the cleaved fragment corresponding to megakaryocyte-potentiating factor (MPF). |
| 使用方法 | WB(1:1000) IHC-P (1:250) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TheMSLN gene encodes a 69 kDa precursor protein that is proteolytically cleaved to yield Megakaryocyte Potentiating Factor (MPF) and a GPI-anchored membrane protein termed mesothelin (1). Expression of (cleaved) mesothelin is largely confined to mesothelial cells of normal pleura, pericardium, and peritoneum, but has been reported to be overexpressed in some cancers, including mesothelioma, and some pancreatic and ovarian adenocarcinomas (1,2). Although suggested to be involved in cell adhesion, the physiological functions of mesothelin have not been determined. It is known, however, that mesothelin can be shed from the cell surface following cleavage by TNF-α converting enzyme. Research studies show that serum levels of mesothelin are markedly increased in patients with mesothelioma and ovarian cancer (1), suggesting that serum mesothelin levels may have utility as a cancer biomarker (1-3). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 46-48, 70 |
| 参考文献 | 1 . Pastan, I. and Hassan, R. (2014) Cancer Res 74, 2907-12. 2 . Villena-Vargas, J. and Adusumilli, P.S. (2012) Ann Cardiothorac Surg 1, 466-71. 3 . Hassan, R. et al. (2004) Clin Cancer Res 10, 3937-42. |
Western blot analysis of extracts from various cell lines using Mesothelin (D9R5G) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded OVCAR8 (left) and PC3 (right) cell pellets using Mesothelin (D9R5G) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human tonsil using Mesothelin (D9R5G) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Mesothelin (D9R5G) XP® Rabbit mAb. | |
Western blot analysis of extracts from OVCAR8 cells, untreated (-) or PNGase F-treated (+), using Mesothelin (D9R5G) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Mesothelin (D9R5G) XP® Rabbit mAb. |
