| 货号 | 98941T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | M |
| 来源宿主 | Rabbit IgG |
| 应用 | W IHC-P |
| 目标/特异性 | CD8α (D4W2Z) XP®Rabbit mAb (Mouse Specific) recognizes endogenous levels of total CD8α protein. |
| 使用方法 | WB(1:1000) IHC-P (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 35-42 |
| 参考文献 | 1 . Zamoyska, R. (1994) Immunity 1, 243-46. |
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD8α (D4W2Z) XP®Rabbit mAb (Mouse Specific). | |
Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD8α (D4W2Z) XP®Rabbit mAb (Mouse Specific). | |
Western blot analysis of extracts from mouse CD8+ T cells, mouse spleen, EL4 cells, and Raw264.7 cells using CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific) (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
