| 货号 | 86163T |
| 同种亚型 | Rabbit |
| 反应种属 | H |
| 来源宿主 | Rabbit |
| 应用 | W IP IHC-P F |
| 目标/特异性 | PD-1 (D4W2J) XP® Rabbit mAb recognizes endogenous levels of total PD-1 protein. |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:200) F (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses (1-3). The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2 (4). Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling (5-7). In addition to activated T-cells, PD-1 is expressed in activated B-cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance (3); however, research studies show that cancer cells often adopt this pathway to escape immune surveillance (9). Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention (10). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 52-65 |
| 参考文献 | 1 . Ishida, Y. et al. (1992) EMBO J 11, 3887-95. 2 . Shinohara, T. et al. (1994) Genomics 23, 704-6. 3 . Nishimura, H. et al. (1999) Immunity 11, 141-51. 4 . Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34. 5 . Yokosuka, T. et al. (2012) J Exp Med 209, 1201-17. 6 . Sheppard, K.A. et al. (2004) FEBS Lett 574, 37-41. 7 . Chemnitz, J.M. et al. (2004) J Immunol 173, 945-54. 8 . Thibult, M.L. et al. (2013) Int Immunol 25, 129-37. 9 . Dong, H. et al. (2002) Nat Med 8, 793-800. 10 . Topalian, S.L. et al. (2012) Curr Opin Immunol 24, 207-12. |
Immunohistochemical analysis of paraffin-embedded 293 cell pellets, control (left) or PD-1 transfected (right), using PD-1 (D4W2J) XP® Rabbit mAb. | |
Western blot analysis of extracts from human CD4+ T cells, MOLT-4, and Jurkat cells using PD-1 (D4W2J) XP® Rabbit mAb (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower). CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of human interleukin-2 (hIL-2) #8907 (6.7 ng/ml). | |
Immunoprecipitation of PD-1 protein from Molt-4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP®Isotype Control #3900, and lane 3 is PD-1 (D4W2J) XP® Rabbit mAb. Western blot analysis was performed using PD-1 | |
(D4W2J) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PD-1 (D4W2J) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkins lymphoma using PD-1 (D4W2J) XP®Rabbit mAb. |
