MTSS1 (D2H4L) XP® Rabbit mAb【科瑞奥博】

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MTSS1 (D2H4L) XP® Rabbit mAb

货号： 93065T 基本售价： 1550.0 元规格： -

产品信息

概述
货号	93065T
同种亚型	Rabbit IgG
反应种属	Human,Rat,
来源宿主	Rabbit IgG
应用	WB, IP , IHC-P , IF-IC
目标/特异性	MTSS1 (D2H4L) XP^® Rabbit mAb recognizes endogenous levels of total MTSS1 protein.
使用方法	WB(1:1000) IP (1:200) IHC-P (1:200) IF-IC (1:1600)

性能
供应商	CST
灵敏度	Endogenous
背景	Metastasis suppressor 1 (MTSS1) is a multi-functional scaffold protein that was initially discovered using a differential display technique that identified proteins missing from bladder cancer cell lines (1,2). MTSS1 (also known as Missing in Metastasis or MIM) is a cytoskeletal remodeling protein that contains a C-terminal WH2 actin-binding motif (1,3). Presence of an IMD (IRSp53/MIM homology) domain allows MTSS1 to induce F-actin bundling and filopodia formation in cells (4). MTSS1 binds to and activates Rac, a protein known to promote the formation of filopodia and lamellipodia (5). The receptor tyrosine phosphatase δ (PTPRD) is associated with MTSS1 and is required for MTSS1-dependent cytoskeletal change (6,7). MTSS1 is a SHH responsive gene that can help regulate GLI-dependent transcriptional activity (8).
运输条件	1
存放说明	-20C
计算分子量	95-105
参考文献	1 . Lee, Y.G. et al. Neoplasia 4, 291-4. 2 . Machesky, L.M. and Johnston, S.A. (2007) J Mol Med 85, 569-76. 3 . Mattila, P.K. et al. (2003) J Biol Chem 278, 8452-9. 4 . Yamagishi, A. et al. (2004) J Biol Chem 279, 14929-36. 5 . Bompard, G. et al. (2005) J Cell Sci 118, 5393-403. 6 . Woodings, J.A. et al. (2003) Biochem J 371, 463-71. 7 . Gonzalez-Quevedo, R. et al. (2005) J Cell Biol 168, 453-63. 8 . Callahan, C.A. et al. (2004) Genes Dev 18, 2724-9.

参考图片

Western blot analysis of extracts from various cell lines using MTSS1 (D2H4L) XP^® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). U-2 OS and HeLa cells are negative for MTSS1 expression, confirming specificity of the antibody.

Confocal immunofluorescent analysis of NCI-H2052 (left), TT (center), and U20S (right) cells using MTSS1 (D2H4L) XP^® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Immunohistochemical analysis of paraffin-embedded TT (left) and U-2 OS (right) cell pellets using MTSS1 (D2H4L) XP^® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human ductal carcinoma of the breast using MTSS1 (D2H4L) XP^® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lymphoid tissue using MTSS1 (D2H4L) XP^® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using MTSS1 (D2H4L) XP^® Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right).

Immunoprecipitation of MTSS1 protein from NCI-H2052 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MTSS1 (D2H4L) XP^® Rabbit mAb. Western blot analysis was performed using MTSS1 (D2H4L) XP^® Rabbit mAb.