| 货号 | 4402T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , F , ChIP |
| 目标/特异性 | FoxP1 (D35D10) XP® Rabbit mAb detects endogenous levels of total FoxP1 protein. |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:100) F (1:50) ChIP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 to FoxP4, which are broadly expressed and play important roles in organ development, immune response, and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region, and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5). |
| 运输条件 | 1 |
| 存放说明 | -20C |
| 计算分子量 | 82-90 |
| 参考文献 | 1 . Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59. 2 . Shu, W. et al. (2001) J Biol Chem 276, 27488-97. 3 . Lu, M.M. et al. (2002) Gene Expr Patterns 2, 223-8. 4 . Koon, H.B. et al. (2007) Expert Opin Ther Targets 11, 955-65. 5 . Li, S. et al. (2004) Mol Cell Biol 24, 809-22. 6 . Wang, B. et al. (2004) Development 131, 4477-87. 7 . Banham, A.H. et al. (2001) Cancer Res 61, 8820-9. 8 . Wlodarska, I. et al. (2005) Leukemia 19, 1299-305. 9 . Goatly, A. et al. (2008) Mod Pathol 21, 902-11. |
Western blot analysis of extracts from various cell lines using FoxP1 (D35D10) XP® Rabbit mAb. 使用FoxP1 (D35D10) XP® Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using FoxP1 (D35D10) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 使用FoxP1 (D35D10) XP® Rabbit mAb对人乳腺癌石蜡包埋组织进行免疫组化，对照多肽（左），抗原特异性多肽（右）。 | |
Flow cytometric analysis of MOLT-4 cells using FoxP1 (D35D10) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 使用FoxP1 (D35D10) XP® Rabbit mAb兔单抗和非特异性阴性对照抗体（红色）对MOLT-4细胞进行流式分析。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Raji cells and either 5 µl of FoxP1 (D35D10) XP® Rabbit mAb, or 2 µl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human BCL2 Promoter Primers #12924, SimpleChIP® Human HSP90B1 Promoter Primers #12899, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
