| 货号 | 4336T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IHC-P , F , IF-IC |
| 目标/特异性 | AML1 (D33G6) XP® Rabbit mAb detects endogenous levels of total AML1 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:1000) F (1:200) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8). |
| 运输条件 | 1 |
| 存放说明 | -20C |
| 计算分子量 | 55 |
| 参考文献 | 1 . Wang, S. et al. (1993) Mol Cell Biol 13, 3324-39. 2 . Ogawa, E. et al. (1993) Proc Natl Acad Sci U S A 90, 6859-63. 3 . Okuda, T. et al. (1996) Cell 84, 321-30. 4 . Wang, Q. et al. (1996) Proc Natl Acad Sci U S A 93, 3444-9. 5 . North, T.E. et al. (2004) Stem Cells 22, 158-68. 6 . Blyth, K. et al. (2005) Nat Rev Cancer 5, 376-87. 7 . Tanaka, T. et al. (1996) Mol Cell Biol 16, 3967-79. 8 . Zhang, Y. et al. (2004) J Biol Chem 279, 53116-25. |
Flow cytometric analysis of Jurkat cells using AML1 (D33G6) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).流式细胞仪分析Jurkat细胞，所用抗体为AML1 (D33G6) XP® Rabbit mAb (蓝色)与非特异性的对照抗体对比 (红色)。 | |
Confocal immunofluorescent analysis of Jurkat cells using AML1 (D33G6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).共聚焦免疫荧光分析Jurkat 细胞，所用抗体为AML1 (D33G6) XP® Rabbit mAb (绿色)。 肌动蛋白微丝用DY-554 phalloidin标记（红色）。 | |
Immunohistochemical analysis of paraffin-embedded human non-Hodgkins lymphoma using AML1 (D33G6) XP® Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).免疫组织化学染色分析石蜡包埋人非霍奇金淋巴瘤组织。在对照多肽 (左图)和抗原特异性多肽 (右图)存在下。所用抗体为AML1 (D33G6) XP® Rabbit mAb 。 | |
Western blot analysis of extracts from various cell lines using AML1 (D33G6) XP® Rabbit mAb.Western免疫印迹。用AML1 (D33G6) XP® Rabbit mAb研究各种细胞系的细胞提取液。 |
