| 货号 | 42427T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , F , ChIP |
| 目标/特异性 | Helios (D8W4X) XP® Rabbit mAb recognizes endogenous levels of total Helios protein. |
| 使用方法 | WB(1:1000) IP (1:200) F (1:100) ChIP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Helios (Ikaros family zinc finger 2, IZKF2) is an Ikaros family transcription factor composed of several zinc fingers that mediate DNA binding and homodimerization or heterodimerization with other Ikaros family proteins (1,2). In the hematopoietic system, Helios expression is restricted to T cells and early hematopoietic progenitors (1,2). In regulatory T cells, expression of Helios contributes to an anergic phenotype by binding to the IL-2 promoter and suppressing IL-2 transcription (3). In addition, alteration of the corresponding Helios gene IZKF2 is one hallmark of low-hypodiploid acute lymphoblastic leukemia (4). |
| 运输条件 | 1 |
| 存放说明 | -20C |
| 计算分子量 | 58, 63-70, 85 |
| 参考文献 | 1 . Hahm, K. et al. (1998) Genes Dev 12, 782-96. 2 . Kelley, C.M. et al. (1998) Curr Biol 8, 508-15. 3 . Baine, I. et al. (2013) J Immunol 190, 1008-16. 4 . Holmfeldt, L. et al. (2013) Nat Genet 45, 242-52. |
Flow cytometric analysis of RL-7 (blue) and Jurkat (green) cells using Helios (D8W4X) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary anitbody. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106Jurkat cells and either 10 μl of Helios (D8W4X) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human IKZF3 Promoter Primers #99663, human IL-2 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Western blot analysis of extracts from various cell lines using Helios (D8W4X) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of Helios from Jurkat cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Helios (D8W4X) XP® Rabbit mAb. Western blot analysis was performed using Helios (D8W4X) XP® Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing Myc-tagged full-length human Helios (hHelios-Myc; +), using Helios (D8W4X) XP® Rabbit mAb. |
