| 货号 | 42078T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P |
| 目标/特异性 | ABCG2 (D5V2K) XP® Rabbit mAb recognizes endogenous levels of total ABCG2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:350) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | ABCG2 (BCRP1/ABCP/MXR) is a member of the ATP-binding cassette transporter family that functions as ATP-dependent transporters for a wide variety of chemical compounds and are associated with drug-resistance in cancer cells (1-6). ABCG2 is a heavily glycosylated transmembrane protein with six transmembrane spanning regions consistent with it functioning as a half-transporter. The ABC family can exist as either full-length transporters or as half-transporters that form functional transporters through homo- or heterodimerization. High expression of ABCG2 was found in placenta as well as cell lines selected for resistance to a number of chemotherapeutic drugs, including mitoxantrone, doxorubicin, topotecan and flavopiridol. In rodents, the highest expression of ABCG2 was found in kidney (8). ABCG2 expression has also been observed in stem cell populations, particularly in hematopoietic and neuronal stem cells and is downregulated with differentiation (9-12). |
| 运输条件 | 1 |
| 存放说明 | -20C |
| 计算分子量 | 65-80 |
| 参考文献 | 1 . Doyle, L.A. and Ross, D.D. (2003) Oncogene 22, 7340-58. 2 . Allen, J.D. et al. (1999) Cancer Res 59, 4237-41. 3 . Doyle, L.A. et al. (1998) Proc Natl Acad Sci U S A 95, 15665-70. 4 . Allikmets, R. et al. (1998) Cancer Res 58, 5337-9. 5 . Miyake, K. et al. (1999) Cancer Res 59, 8-13. 6 . Robey, R.W. et al. (2001) Clin Cancer Res 7, 145-52. 7 . Zhou, S. et al. (2001) Nat Med 7, 1028-34. 8 . Honscha, W. et al. (2000) Hepatology 31, 1296-304. 9 . Scharenberg, C.W. et al. (2002) Blood 99, 507-12. 10 . Islam, M.O. et al. (2005) Neurosci Res 52, 75-82. 11 . Bunting, K.D. (2002) Stem Cells 20, 11-20. 12 . Scharenberg, C.W. et al. (2002) Blood 99, 507-12. |
Immunohistochemical analysis of paraffin-embedded human placenta using ABCG2 (D5V2K) XP® Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded normal human uterus using ABCG2 (D5V2K) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded A549 (left) and A-204 (right) cell pellets using ABCG2 (D5V2K) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using ABCG2 (D5V2K) XP® Rabbit mAb. | |
Western blot analysis of extracts from RPMI 8226 and A549 cells and human placenta using ABCG2 (D5V2K) XP® Rabbit mAb. | |
Immunoprecipitation of ABCG2 from A549 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is ABCG2 (D5V2K) XP® Rabbit mAb. Western blot analysis was performed using ABCG2 (D5V2K) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse kidney using ABCG2 (D5V2K) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse liver using ABCG2 (D5V2K) XP® Rabbit mAb. |
