| 货号 | 3366T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , IF-F |
| 目标/特异性 | Neuropilin-2 (D39A5) XP® Rabbit mAb detects endogenous levels of total neuropilin-2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) IF-F (1:2000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single pass, transmembrane signaling proteins encompassing Plexin A1, A2, A3 and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3).Neuropilin-2, a single pass, transmembrane glycoprotein, can also bind to VEGF165 and VEGF145, but not VEGF121. Thus, in addition to neuronal guidance, neuropilin-2 plays a role in angiogenesis and cardiovascular functions (4). Although neuropilin-2 has been linked to tumor biology (e.g. pancreatic cancer, bladder cancer, lung cancer, and neuroblastoma), its role in tumor pathogenesis is still under investigation (4,5). |
| 运输条件 | 1 |
| 存放说明 | -20C |
| 计算分子量 | 130 |
| 参考文献 | 1 . Pasterkamp, R.J. and Kolodkin, A.L. (2003) Curr Opin Neurobiol 13, 79-89. 2 . Takahashi, T. and Strittmatter, S.M. (2001) Neuron 29, 429-39. 3 . Zanata, S.M. et al. (2002) J Neurosci 22, 471-7. 4 . Favier, B. et al. (2006) Blood 108, 1243-50. 5 . Chen, C. et al. (2005) World J Surg 29, 271-5. |
Western blot analysis of extracts from rat brain and NIH/3T3 cells using Neuropilin-2 (D39A5) XP® Rabbit mAb. Western blot分析大鼠大脑和NIH/3T3细胞提取物，使用的抗体是Neuropilin-2 (D39A5) XP® Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded mouse pancreas using Neuropilin-2 (D39A5) XP® Rabbit mAb. 免疫组化分析石蜡包埋的小鼠胰腺组织，使用的抗体是Neuropilin-2 (D39A5) XP® Rabbit mAb 兔单抗。 | |
Confocal immunofluorescent analysis of E14.5 mouse embryo using Neuropilin-2 (D39A5) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析E14.5小鼠胚胎，使用的抗体是Neuropilin-2 (D39A5) XP® Rabbit mAb 兔单抗（绿色）。蓝色伪彩=DRAQ5 ®#4084(荧光DNA染料)。 | |
Confocal immunofluorescent analysis of rat pancreas using Neuropilin2 (D39A5) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析大鼠胰腺，使用的抗体是Neuropilin2 (D39A5) XP® Rabbit mAb 兔单抗（绿色）。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 |
