| 货号 | 32934T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , F , IF-IC |
| 目标/特异性 | Annexin A1 (D5V2T) XP® Rabbit mAb recognizes endogenous levels of total annexin A1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:400) F (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The annexin superfamily consists of 13 calcium or calcium and phospholipid binding proteins with high biological and structural homology (1). Annexin-1 (ANXA1) is the first characterized member of the annexin family of proteins and is able to bind to cellular membranes in a calcium-dependent manner, promoting membrane fusion and endocytosis (2-4). Annexin A1 has anti-inflammatory properties and inhibits phospholipase A2 activity (5,6). Annexin A1 can accumulate on internalized vesicles after EGF-stimulated endocytosis and may be required for a late stage in inward vesiculation (7). Phosphorylation by PKC, EGFR, and Chak1 results in inhibition of annexin A1 function (8-10). Annexin A1 has also been identified as one of the eat-me signals on apoptotic cells that are to be recognized and ingested by phagocytes (11). Annexin A1, as an endogenous anti-inflammatory mediator, has roles in many diverse cellular functions, such as membrane aggregation, inflammation, phagocytosis, proliferation, apoptosis, and tumorigenesis and cancer development (12-14). |
| 运输条件 | 1 |
| 存放说明 | -20C |
| 计算分子量 | 38 |
| 参考文献 | 1 . Raynal, P. and Pollard, H.B. (1994) Biochim Biophys Acta 1197, 63-93. 2 . Blackwell, G.J. et al. (1980) Nature 287, 147-9. 3 . Rothhut, B. et al. (1983) Biochem Biophys Res Commun 117, 878-84. 4 . Hirata, F. et al. (1981) Proc Natl Acad Sci USA 78, 3190-4. 5 . Kim, K.M. et al. (1994) FEBS Lett 343, 251-5. 6 . Kim, S.W. et al. (2001) J Biol Chem 276, 15712-9. 7 . White, I.J. et al. (2006) EMBO J 25, 1-12. 8 . Varticovski, L. et al. (1988) Biochemistry 27, 3682-90. 9 . Dorovkov, M.V. and Ryazanov, A.G. (2004) J Biol Chem 279, 50643-6. 10 . Wang, W. and Creutz, C.E. (1994) Biochemistry 33, 275-82. 11 . Arur, S. et al. (2003) Dev Cell 4, 587-98. 12 . Perretti, M. and Gavins, F.N. (2003) News Physiol Sci 18, 60-4. 13 . Parente, L. and Solito, E. (2004) Inflamm Res 53, 125-32. 14 . Lim, L.H. and Pervaiz, S. (2007) FASEB J 21, 968-75. |
Confocal immunofluorescent analysis of A549 (positive, left) and 293T (negative, right) cells using Annexin A1 (D5V2T) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of RL7 cells (blue) and Jurkat cells (green) using Annexin A1 (D5V2T) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Western blot analysis of extracts from various cell lines using Annexin A1 (D5V2T) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of Annexin A1 from A549 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Annexin A1 (D5V2T) XP® Rabbit mAb. Western blot analysis was performed using Annexin A1 (D5V2T) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded A549 (left) and LNCaP (right) cell pellets using Annexin A1 (D5V2T) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human spleen using Annexin A1 (D5V2T) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma of the ovary using Annexin A1 (D5V2T) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded normal human colon using Annexin A1 (D5V2T) XP® Rabbit mAb. |
