| 货号 | 18381T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | HSL (D6W5S) XP® Rabbit mAb recognizes endogenous levels of total HSL protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | HSL (hormone-sensitive lipase) catalyzes the hydrolysis of triacylglycerol, the rate-limiting step in lipolysis. Lipolytic stimuli activate adenylyl cyclase and thus increase intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA phosphorylates HSL at Ser563, Ser659, and Ser660, which stimulates HSL activity (1,2). In contrast, AMPK phosphorylates HSL at Ser565, which reduces HSL phosphorylation at Ser563 by PKA and inhibits HSL activity (2,3). Recent work indicates that phosphorylation at Ser600 by p44/42 MAPKs also enhances the enzymatic activity of HSL (4). |
| 运输条件 | 1 |
| 存放说明 | -20C |
| 计算分子量 | 81, 83 |
| 参考文献 | 1 . Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-37. 2 . Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-21. 3 . Garton, A.J. and Yeaman, S.J. (1990) Eur. J. Biochem. 191, 245-50. 4 . Greenberg, A.S. et al. (2001) J. Biol. Chem. 276, 45456-61. |
Confocal immunofluorescent analysis of differentiated human adipocytes (left) or human pre-adipocytes (right) using HSL (D6W5S) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight® 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from human pre-adipocytes and differentiated human adipocytes using HSL (D6W5S) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of HSL from differentiated human adipocyte extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is HSL (D6W5S) XP® Rabbit mAb. Western blot analysis was performed using | |
HSL (D6W5S) XP® Rabbit mAb. |
