| 货号 | 12709T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IHC-P , F , IF-IC |
| 目标/特异性 | PAX5 (D7H5X) XP® Rabbit mAb recognizes endogenous levels of total PAX5 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:50) F (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintains functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2). |
| 运输条件 | 1 |
| 存放说明 | -20C |
| 计算分子量 | 45 |
| 参考文献 | 1 . Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14. 2 . Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62. 3 . Wang, Q. et al. (2008) J Cell Mol Med 12, 2281-94. 4 . Blake, J.A. et al. (2008) Dev Dyn 237, 2791-803. 5 . Cobaleda, C. et al. (2007) Nat Immunol 8, 463-70. 6 . Busslinger, M. (2004) Annu Rev Immunol 22, 55-79. 7 . Carotta, S. and Nutt, S.L. (2008) Bioessays 30, 203-7. 8 . Heltemes-Harris, L.M. et al. (2011) J Exp Med 208, 1135-49. |
Confocal immunofluorescent analysis of Ramos (left) and Jurkat (right) cells using PAX5 (D7H5X) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
使用PAX5 (D7H5X) XP® Rabbit mAb（绿色）对Ramos（左）和Jurkat（右）细胞进行共聚焦免疫荧光分析。肌动蛋白丝使用DY-554 phalloidin标记（红色）。蓝色伪彩= DRAQ5® #4084 (DNA荧光染色). | |
Western blot analysis of extracts from various cell lines using PAX5 (D7H5X) XP® Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). 使用PAX5 (D7H5X) XP® 兔mAb（上）和β-actin (D6A8)Rabbit mAb #8457对多种细胞提取物进行western blot分析。 |
