| 货号 | 36746T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | IP, F , IF-IC |
| 目标/特异性 | EpCAM (D4K8R) XP® Rabbit mAb recognizes endogenous levels of total EpCAM protein. |
| 使用方法 | IP IF-IC F |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). Research studies identified EpCAM as one of the first tumor-associated antigens, and it has long been a marker of epithelial and tumor tissue. Investigators have shown that EpCAM is highly expressed in cancer cells (reviewed in 2,3). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
| 参考文献 | 1 . Went, P.T. et al. (2004) Hum Pathol 35, 122-8. 2 . Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23. 3 . Armstrong, A. and Eck, S.L. (2003) Cancer Biol Ther 2, 320-6. |
Flow cytometric analysis of fixed and unpermeabilized (left) and live (right) HeLa cells (blue), HT-29 cells (red), and MCF7 cells (green) using EpCAM (D4K8R) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Confocal immunofluorescent analysis of HT-29 (left) and HeLa (right) cells using EpCAM (D4K8R) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Note that EpCAM (D4K8R) XP® Rabbit mAb has been shown to detect its target protein on live, fixed, and fixed/permeabilized cells. |
