| 货号 | 11976T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-F/IF-IC |
| 目标/特异性 | Neuropeptide Y (D7Y5A) XP® Rabbit mAb recognizes endogenous levels of precursor (11 kDa) and mature (4 kDa) neuropeptide Y. This antibody does not cross-react with peptide YY or peptide YY (3-36) peptides. This antibody may cross-react with a protein of unknown origin at 80 kDa in some cell lines. |
| 使用方法 | WB(1:1000) IHC-P (1:100) IF-F (1:3200) IF-IC (1:3200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Neuropeptide Y (NPY) is a 36 amino acid peptide that belongs to the pancreatic polypeptide (PP) family, which also includes peptide YY (PYY) (1). The mature 36-residue NPY is produced from a larger pre-pro 97-residue NPY precursor through a series of cleavage reactions at dibasic sites and C-terminal amidation of the peptide product (2). NPY is widely expressed in the central nervous system (3) and exerts its action through stimulation of 5 different receptors (Y1-Y5) that belong to the G protein-coupled receptor family (4). NPY in the hypothalamus exhibits orexigenic activity through activation of Y1 and Y5 receptors (5). NPY is involved in the control of bone homeostasis, through the regulation of osteoblast activity by Y1 and Y2 receptors (6), and the regulation of testosterone secretion by activating Y1 receptor in testicular vessels (7). Research studies suggest that modulation of NPY activity and signaling represents a potential strategy for the development of appetite control and antiobesity agents (8). |
| 存放说明 | -20C |
| 计算分子量 | 4, 11 |
| 参考文献 | 1 . Tatemoto, K. et al. (1982) Nature 296, 659-60. 2 . OHare, M.M. and Schwartz, T.W. (1989) Cancer Res 49, 7010-4. 3 . Adrian, T.E. et al. (1983) Nature 306, 584-6. 4 . Larhammar, D. and Salaneck, E. (2004) Neuropeptides 38, 141-51. 5 . Lin, S. et al. (2004) Neuropeptides 38, 189-200. 6 . Lee, N.J. and Herzog, H. (2009) Neuropeptides 43, 457-63. 7 . Allen, C.D. et al. (2011) Neuropeptides 45, 55-61. 8 . Simpson, K.A. et al. (2009) Arq Bras Endocrinol Metabol 53, 120-8. |
Confocal immunofluorescent analysis of SH-SY5Y cells, treated with TPA #4174 (200 nM, 24 hr; left) or untreated (right), using Neuropeptide Y (D7Y5A) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析SH-SY5Y细胞、TPA #4174 （200 nM, 24 hr; 左图）处理或未处理（右图），使用的抗体是Neuropeptide Y (D7Y5A) XP® Rabbit mAb兔单抗（绿色），肌动蛋白纤丝用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Confocal immunofluorescent analysis of adult rat retina using Neuropeptide Y (D7Y5A) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析成年大鼠视网膜，使用的抗体是Neuropeptide Y (D7Y5A) XP® Rabbit mAb兔单抗（绿色）。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Neuropeptide Y (D7Y5A) XP® Rabbit mAb. 免疫组化分析石蜡包埋的人结乳腺癌切片，使用的抗体是Neuropeptide Y (D7Y5A) XP® Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from SH-SY5Y cells, untreated (-) or treated with TPA #4174 (200 nM, 24 hr; +), using Neuropeptide Y (D7Y5A) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Western blot 检测 SH-SY5Y 细胞提取物，未处理 (-)或 TPA #4174 (200 nM, 24 hr; +)处理，使用的抗体是Neuropeptide Y (D7Y5A) XP® Rabbit mAb兔单抗（上图）或β-Actin (D6A8) Rabbit mAb兔单抗 #8457（下图）。 | |
Western blot analysis of the indicated amounts of human peptide YY (PYY), peptide YY (PYY) (3-36), and neuropeptide Y (NPY) peptides using Neuropeptide Y (D7Y5A) XP® Rabbit mAb (upper). Peptide loading was confirmed by staining overnight with Coomassie Blue (lower). Western blot 定量检测人肽YY(PYY)、肽YY (PYY) (3-36)和神经肽Y（NPY），使用的抗体是Neuropeptide Y (D7Y5A) XP® Rabbit mAb兔单抗（上图）。使用考马斯亮蓝过夜染色验证内参肽（下图）。 | |
Western blot analysis of the indicated amounts of human neuropeptide Y using Neuropeptide Y (D7Y5A) XP® Rabbit mAb. Western blot 定量检测人神经肽Y，使用的抗体是Neuropeptide Y (D7Y5A) XP® Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from human hypothalamus and rat spleen using Neuropeptide Y (D7Y5A) XP® Rabbit mAb. Western blot 检测人体下丘脑和大鼠脾脏提取物，使用的抗体是Neuropeptide Y (D7Y5A) XP® Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded mouse brain using Neuropeptide Y (D7Y5A) XP® Rabbit mAb. 免疫组化分析石蜡包埋的小鼠大脑切片，使用的抗体是Neuropeptide Y (D7Y5A) XP® Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded rat brain using Neuropeptide Y (D7Y5A) XP® Rabbit mAb. |
