| 货号 | 12120T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/ChIP |
| 目标/特异性 | Bcl-11B (D6F1) XP® Rabbit mAb recognizes endogenous levels of total Bcl-11B protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:200) ChIP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Bcl-11B (Ctip2) is a COUP-TF interacting protein that belongs to the C2H2 type zinc finger protein family (1). Bcl-11B is highly expressed in the brain and is critical for the development of neurons, as well as other tissues and organs. Bcl-11B also plays an essential role in T cell lineage commitment and maintenance of T cell identity (1-3). Two isoforms of Bcl-11B are found to be encoded by the BCL11B gene, possibly through exon-skipping (4). Bcl-11B is a transcription factor which binds to target genes through the 2nd and 3rd zinc-finger domains of exon 4 (3), while also interacting with various protein partners including COUP-TF proteins (1), the NuRD complex (5,6), HDAC1, HDAC2, and SUV39H1 (7). Research studies have shown that mutations and deletion of Bcl-11B contribute to the development of thymic lymphoma in mice and T cell acute lymphoblastic leukemia in humans, indicating a role as a tumor suppressor (4,8). Mechanistic studies have shown that Bcl-11B represses gene expression of the E3 ubiquitin ligase HDM2 in a p53-dependent manner (9). |
| 存放说明 | -20C |
| 计算分子量 | 120-130 |
| 参考文献 | 1 . Avram, D. et al. (2000) J Biol Chem 275, 10315-22. 2 . Liu, P. et al. (2010) Immunol Rev 238, 138-49. 3 . Kominami, R. (2012) Proc Jpn Acad Ser B Phys Biol Sci 88, 72-87. 4 . Wakabayashi, Y. et al. (2003) Biochem Biophys Res Commun 301, 598-603. 5 . Cismasiu, V.B. et al. (2005) Oncogene 24, 6753-64. 6 . Topark-Ngarm, A. et al. (2006) J Biol Chem 281, 32272-83. 7 . Marban, C. et al. (2007) EMBO J 26, 412-23. 8 . Gutierrez, A. et al. (2011) Blood 118, 4169-73. 9 . Obata, M. et al. (2012) Cell Signal 24, 1047-52. |
Confocal immunofluorescent analysis of Jurkat (positive; left) and HeLa (negative; right) cells using Bcl-11B (D6F1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
使用Bcl-11B (D6F1) XP®Rabbit mAb（绿色）对Jurkat（阳性；左）和HeLa（阴性；右）细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DY-554 phalloidin 标记（红色）。蓝色伪彩= DRAQ5® #4084 (DNA荧光染色)。 | |
