| 货号 | 11998T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | SDHA (D6J9M) XP® Rabbit mAb recognizes endogenous levels of total SDHA protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Succinate dehydrogenase (SDH), also known as Complex II or succinate:quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (1). Specifically, it is involved in the oxidation of succinate (2). SDH consists of four subunits: SDHA, SDHB, SDHC, and SDHD (3). Research studies have shown that defects in SDHA cause complex II deficiency (2). In addition, investigators have observed reduction of SDHA in the striatum of patients with Huntington’s disease (3), and reduction of SDHB, SDHC, and SDHD in paragangliomas and phenochromocytomas(4,5). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
| 参考文献 | 1 . Oyedotun, K.S. and Lemire, B.D. (2004) J Biol Chem 279, 9424-31. 2 . Bourgeron, T. et al. (1995) Nat Genet 11, 144-9. 3 . Benchoua, A. et al. (2006) Mol Biol Cell 17, 1652-63. 4 . Baysal, B.E. et al. (2000) Science 287, 848-51. 5 . Feichtinger, R.G. et al. (2010) BMC Cancer 10, 149. |
Confocal immunofluorescent analysis of HeLa cells using SDHA (D6J9M) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析293细胞，使用抗体为SDHA (D6J9M) XP® Rabbit mAb兔单抗（绿色）和β-Actin (8H10D10) Mouse mAb鼠单抗#3700（红色）。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Western blot analysis of extracts from various cell lines using SDHA (D6J9M) XP® Rabbit mAb. Wetern blot检测多种细胞系提取物，所用抗体为SDHA (D6J9M) XP® Rabbit mAb兔单抗。 | |
Immunoprecipitation of SDHA from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SDHA (D6J9M) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SDHA (D6J9M) XP® Rabbit mAb. 免疫沉淀方法检测HeLa细胞提取物SDHA蛋白，使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或SDHA (D6J9M) XP® Rabbit mAb兔单抗 (泳道3)。泳道1为10%对照。使用SDHA (D6J9M) XP® Rabbit mAb兔单抗 进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SDHA (D6J9M) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 免疫组织化学分析石蜡包埋的人结肠癌切片，使用抗体为SDHA (D6J9M) XP® Rabbit mAb兔单抗，对照（左）或抗原特异性肽（右）。 |
