| 货号 | 30036T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IHC-P |
| 目标/特异性 | β-Arrestin (D7Z3W) XP® Rabbit mAb recognizes endogenous levels of total β-arrestin 1 protein. This antibody does not cross-react with other arrestin proteins. |
| 使用方法 | WB(1:1000) IHC-P (1:300) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Arrestin proteins function as negative regulators of G protein-coupled receptor (GPCR) signaling. Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylated GPCR and the eventual internalization of the receptor and desensitization of GPCR signaling (1). Four distinct mammalian arrestin proteins are known. Arrestin 1 (also known as S-arrestin) and arrestin 4 (X-arrestin) are localized to retinal rods and cones, respectively. Arrestin 2 (also known as β-arrestin 1) and arrestin 3 (β-arrestin 2) are ubiquitously expressed and bind to most GPCRs (2). β-arrestins function as adaptor and scaffold proteins and play important roles in other processes, such as recruiting c-Src family proteins to GPCRs in Erk activation pathways (3,4). β-arrestins are also involved in some receptor tyrosine kinase signaling pathways (5-8). Additional evidence suggests that β-arrestins translocate to the nucleus and help regulate transcription by binding transcriptional cofactors (9,10). |
| 存放说明 | -20C |
| 计算分子量 | 51 |
| 参考文献 | 1 . Shenoy, S.K. and Lefkowitz, R.J. (2005) Sci STKE 2005, cm10. 2 . Lefkowitz, R.J. and Shenoy, S.K. (2005) Science 308, 512-7. 3 . Luttrell, L.M. et al. (1999) Science 283, 655-61. 4 . Luttrell, L.M. et al. (1999) Curr Opin Cell Biol 11, 177-83. 5 . Luttrell, L.M. and Lefkowitz, R.J. (2002) J Cell Sci 115, 455-65. 6 . Waters, C. et al. (2004) Semin Cell Dev Biol 15, 309-23. 7 . Lefkowitz, R.J. and Whalen, E.J. (2004) Curr Opin Cell Biol 16, 162-8. 8 . Waters, C.M. et al. (2005) Cell Signal 17, 263-77. 9 . Kang, J. et al. (2005) Cell 123, 833-47. 10 . Ma, L. and Pei, G. (2007) J Cell Sci 120, 213-8. 11 . Oakley, R.H. et al. (2012) Proc Natl Acad Sci U S A 109, 17591-6. |
Immunohistochemical analysis of paraffin-embedded A549 cell pellets, untreated (left) or dexamethasone-treated (100nM, 16 hr; right), using β-Arrestin 1 (D7Z3W) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using β-Arrestin 1 (D7Z3W) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using β-Arrestin 1 (D7Z3W) XP® Rabbit mAb. | |
Western blot analysis of extracts from A549 cells, vehicle-treated (-) or treated with dexamethasone (100 nM, 16 hr; +), using β-Arrestin 1 (D7Z3W) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from various cell lines using β-Arrestin 1 (D7Z3W) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
