| 货号 | 12389T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-F |
| 目标/特异性 | GFAP (D1F4Q) XP® Rabbit mAb recognizes endogenous levels of total GFAP protein. |
| 使用方法 | WB(1:1000) IF-F (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are specifically expressed in particular cell types: cytokeratins in epithelial cells, glial fibrillary acidic protein (GFAP) in glial cells, desmin in skeletal, visceral, and certain vascular smooth muscle cells, vimentin in cells of mesenchymal origin, and neurofilaments in neurons. GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). In addition, GFAP intermediate filaments are also present in nonmyelin-forming Schwann cells in the peripheral nervous system (3). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
| 参考文献 | 1 . Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51. 2 . Goebel, H.H. et al. (1987) Acta Histochem Suppl 34, 81-93. 3 . Jessen, K.R. et al. (1990) Development 109, 91-103. |
Confocal immunofluorescent analysis of rat cerebellum using GFAP (D1F4Q) XP® Rabbit mAb (green) and Neurofilament-H (RMdO 20) Mouse mAb #2836 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析大鼠小脑，使用的抗体是GFAP (D1F4Q) XP® Rabbit mAb兔单抗（绿色）和Neurofilament-H (RMdO 20) Mouse mAb 鼠单抗#2836（红色）。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Western blot analysis of extracts from mouse brain, NIH/3T3 cells, rat brain, and C6 cells using GFAP (D1F4Q) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Western blot检测小鼠大脑、NIH/3T3细胞、大鼠大脑和C6细胞提取物，使用的抗体是GFAP (D1F4Q) XP® Rabbit mAb兔单抗（上图）或β-Actin (D6A8) Rabbit mAb兔单抗 #8457（下图）。 |
