| 货号 | 12295T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | FXR1 (D10A2) XP® Rabbit mAb recognizes endogenous levels of total FXR1 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Fragile X syndrome is a genetic disorder characterized by a spectrum of physical and behavioral features and is a frequent form of inherited mental retardation (1). X-linked FMRP (FMR-1) and its two autosomal homologs, FXR1 and FXR2, are polyribosome-associated RNA-binding proteins that are involved in the pathogenesis of fragile X syndrome (1-3). Each of the fragile X proteins can self-associate, as well as form heteromers with the other two related proteins (3). FMRP can act as a translation regulator and is a component of RNAi effector complexes (RISC), suggesting a role in gene silencing (4). The Drosophila homolog of FMRP (dFMRP) associates with Argonaute 2 (Ago2) and Dicer and can coimmunoprecipitate with miRNA and siRNA (5). These results suggest that fragile X syndrome is related to abnormal translation caused by defects in RNAi-related pathways. In addition, FMRP, FXR1, and FXR2 are components of stress granules (SG) and have been implicated in the translational regulation of mRNAs (6). |
| 存放说明 | -20C |
| 计算分子量 | 78-80, 82-84 |
| 参考文献 | 1 . Verkerk, A.J. et al. (1991) Cell 65, 905-14. 2 . Siomi, M.C. et al. (1995) EMBO J 14, 2401-8. 3 . Zhang, Y. et al. (1995) EMBO J 14, 5358-66. 4 . Caudy, A.A. et al. (2002) Genes Dev 16, 2491-6. 5 . Siomi, H. et al. (2004) Ment Retard Dev Disabil Res Rev 10, 68-74. 6 . Linder, B. et al. (2008) Hum Mol Genet 17, 3236-46. |
Confocal immunofluorescent analysis of C2C12 cells, untreated (left) or MG-132 treated (10 μg/mL, 3 hr; right), using FXR1 (D10A2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 采用共聚焦免疫荧光术分别检测未处理（左）或MG-132 处理 (10 μg/mL, 3小时; 右)的 C2C12细胞，使用的抗体为 FXR1 (D10A2) XP® Rabbit mAb兔单抗 (绿色)。肌动蛋白微丝使用DY-554 phalloidin进行标记（红色）。蓝色伪彩= DRAQ5® #4084 (荧光DNA染料)。 | |
Western blot analysis of extracts from various cell lines using FXR1 (D10A2) XP® Rabbit mAb. Western blot方法检测多种细胞系的提取物，使用的抗体为 FXR1 (D10A2) XP® Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from human and mouse skeletal muscle tissues using FXR1 (D10A2) XP® Rabbit mAb. Western blot方法检测人和小鼠骨骼肌的组织提取物，使用的抗体为 FXR1 (D10A2) XP® Rabbit mAb兔单抗。 |
