| 货号 | 12238T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Calreticulin (D3E6) XP® Rabbit mAb recognizes endogenous levels of total calreticulin protein. |
| 使用方法 | WB(1:1000) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Calcium is a universal signaling molecule involved in many cellular functions such as cell motility, metabolism, protein modification, protein folding, and apoptosis. Calcium is stored in the endoplasmic reticulum (ER), where it is buffered by calcium binding chaperones such as calnexin and calreticulin, and is released via the IP3 Receptor channel (1). Calreticulin also functions as an ER chaperone that ensures proper folding and quality control of newly synthesized glycoproteins. As such, calreticulin presumably does not alter protein folding but regulates proper timing for efficient folding and subunit assembly. Furthermore, calreticulin retains proteins in non-native conformation within the ER and targets them for degradation (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
| 参考文献 | 1 . Groenendyk, J. et al. (2004) Mol Cells 17, 383-9. 2 . Nauseef, W.M. et al. (1995) J Biol Chem 270, 4741-7. 3 . Williams, D.B. (2006) J. Cell Sci. 119, 615-623. |
Confocal immunofluorescent analysis of NIH/3T3 cells using Calreticulin (D3E6) XP® Rabbit mAb (green) and β-Actin (13E5) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8046 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦荧光法检测NIH/3T3 细胞，使用的抗体为Calreticulin (D3E6) XP® Rabbit mAb (绿色) 和 β-Actin (13E5) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8046 (红色).蓝色伪彩色为DNA荧光染料（产品信息为DRAQ5® #4084）。 | |
Western blot analysis of extracts from SH-SY5Y and KNRK cells using Calreticulin (D3E6) XP® Rabbit mAb. Western blot方法检测SH-SY5Y 和KNRK细胞提取物，使用的抗体为Calreticulin (D3E6) XP® Rabbit mAb. |
