| 货号 | 12503T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Caldesmon-1 (D5C8D) XP® Rabbit mAb recognizes endogenous levels of total caldesmon-1 protein. Based on sequence homology, the antibody is expected to cross-react with both the smooth muscle and nonmuscle isoforms. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Caldesmon-1 is an actin filament stabilizing protein involved in the regulation of cell contraction. Binding of caldesmon-1 to actin is weakened by phosphorylation and by calmodulin in the presence of calcium. Caldesmon-1 is encoded by a single gene, which is spliced to generate a widely distributed low molecular weight form and a smooth muscle specific high molecular weight form (1,2). Caldesmon-1 is phosphorylated by the cyclin dependent kinase cdc2 and Erk1/2 MAP kinase, both of which prevent the activity of caldesmon-1 (3-5). Phosphorylation of caldesmon-1 by cdc2 is required for passage of cells through mitosis (6). Phosphorylation by Erk1/2 is important in regulating smooth muscle contraction (7). Caldesmon-1 activity may play a role in the formation of podosomes, adhesion complexes associated with the secretion of matrix metalloproteases, invasion, and metastasis (reviewed in 5). |
| 存放说明 | -20C |
| 计算分子量 | 70-80 nonmuscle, 120-150 smooth muscle |
| 参考文献 | 1 . Hayashi, K. et al. (1992) Proc. Natl. Acad. Sci. USA 89, 12122-12126. 2 . Humphrey, M.B. et al. (1992) Gene 112, 197-204. 3 . Yamashiro, S. et al. (1991) Nature 349, 169-172. 4 . Mak, A.S. et al. (1991) J. Biol. Chem. 266, 6678-6681. 5 . Hai, C.M. and Gu, Z. (2006) Eur. J. Cell Biol. 85, 305-309. 6 . Yamashiro, S. et al. (2001) Mol. Biol. Cell 12, 239-250. 7 . Hedges, J.C. et al. (2000) Am. J. Physiol. Cell Physiol. 278, C718-C7126. |
Confocal immunofluorescent analysis of HeLa (left) and MCF7 (right) cells using Caldesmon-1 (D5C8D) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Caldesmon-1 (D5C8D) XP® Rabbit mAb（绿色）对HeLa（左）和MCF7（右）细胞进行激光共聚焦免疫荧光分析。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using Caldesmon-1 (D5C8D) XP® Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).使用Caldesmon-1 (D5C8D) XP® Rabbit mAb（上）或α-Actinin (D6F6) XP® Rabbit mAb #6487（下）对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of caldesmon-1 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Caldesmon-1 (D5C8D) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Caldesmon-1 (D5C8D) XP® Rabbit mAb.使用 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或Caldesmon-1 (D5C8D) XP®兔mAb（lane3）对HeLa细胞中的Caldesmon-1 进行免疫沉淀实验。Lane1是10% 的上样量。使用Caldesmon-1 (D5C8D) XP® Rabbit mAb进行western blot分析。 |
