Cathepsin B (D1C7Y) XP® Rabbit mAb【科瑞奥博】

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Cathepsin B (D1C7Y) XP® Rabbit mAb

货号： 31718T 基本售价： 1550.0 元规格： -

产品信息

概述
货号	31718T
同种亚型	Rabbit IgG
反应种属	Human,Mouse,Rat,
来源宿主	Rabbit IgG
应用	WB, IHC-P , F , IF-IC
目标/特异性	Cathepsin B (D1C7Y) XP^® Rabbit mAb recognizes endogenous levels of total cathepsin B protein. This antibody detects the heavy chain subunit of cathepsin B.
使用方法	WB(1:1000) IHC-P (1:2000) F (1:100) IF-IC (1:800)

性能
供应商	CST
灵敏度	Endogenous
背景	Cathepsin B (CSTB), part of the papain family of proteases, is a widely expressed lysosomal cysteine endopeptidase (1,2). Cathepsin B is produced from a larger precursor form, pro-cathepsin B, which runs at approximately 44 kDa on SDS-PAGE, and is proteolytically processed and glycosylated to form a mature two-chain protein containing a heavy chain (running at 27 and 24 kDa) and a light chain (5 kDa). High levels of cathepsin B are found in macrophages and osteoclasts, as well as various types of cancer cells, including lung, colon, prostate, breast, and stomach. In addition, expression of cathepsin B has been associated with multiple sclerosis (3), rheumatoid arthritis (4), and pancreatitis (5). While generally localized to lysosomes, in cancer alterations can lead to its secretion (6). Its role in tumor progression is thought to involve promotion of basement membrane degradation, invasion and metastasis (7,8). Expression can correlate with poor prognosis for a variety of forms of cancer (9-13).
存放说明	-20C
计算分子量	44, 27, 24
参考文献	1 . Chan, S.J. et al. (1986) Proc Natl Acad Sci USA 83, 7721-5. 2 . Fong, D. et al. (1986) Proc Natl Acad Sci USA 83, 2909-13. 3 . Bever, C.T. et al. (1994) Neurology 44, 745-8. 4 . Hashimoto, Y. et al. (2001) Biochem Biophys Res Commun 283, 334-9. 5 . Halangk, W. et al. (2000) J Clin Invest 106, 773-81. 6 . Berquin, I.M. and Sloane, B.F. (1996) Adv Exp Med Biol 389, 281-94. 7 . Yan, S. et al. (1998) Biol Chem 379, 113-23. 8 . Vasiljeva, O. et al. (2006) Cancer Res 66, 5242-50. 9 . Campo, E. et al. (1994) Am J Pathol 145, 301-9. 10 . Foekens, J.A. et al. (1998) J Clin Oncol 16, 1013-21. 11 . Werle, B. et al. (1999) Br J Cancer 81, 510-9. 12 . Lah, T.T. et al. (2000) Clin Cancer Res 6, 578-84. 13 . Werle, B. et al. (2000) Cancer 89, 2282-91.

参考图片

Immunohistochemical analysis of paraffin-embedded normal human kidney using Cathepsin B (D1C7Y) XP(R) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Cathepsin B (D1C7Y) XP(R) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Cathepsin B (D1C7Y) XP(R) Rabbit mAb.

Western blot analysis of extracts from various cell lines using Cathepsin B (D1C7Y) XP^® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing full-length human Cathepsin B (hCTSB; +) or mouse Cathespin B (mCTSB; +) using Cathepsin B (D1C7Y) XP^® Rabbit mAb.

Confocal immunofluorescent analysis of Malme-3M (left) and MCF7 (right) cells using Cathepsin (D1C7Y) XP^® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Flow cytometric analysis of Daudi cells (blue) and U266 cells (green) using Cathepsin B (D1C7Y) XP^® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.