| 货号 | 12698T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F/ChIP |
| 目标/特异性 | His-Tag (D3I1O) XP® Rabbit mAb recognizes recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:800) IF-IC (1:400) ChIP (1:100) |
| 供应商 | CST |
| 灵敏度 | Transfected Only |
| 背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged proteins biochemical properties. |
| 存放说明 | -20C |
| 参考文献 | 1 . Kroll, D.J. et al. (1993) DNA Cell Biol 12, 441-53. 2 . di Guan, C. et al. (1988) Gene 67, 21-30. |
Confocal immunofluorescent analysis of 293T cells transfected with a His-Tagged protein using His-Tag (D3I1O) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析293T细胞，转染了His标签蛋白，使用的抗体是：His-Tag (D3I1O) XP® Rabbit mAb（绿色）。肌动蛋白纤维用DY-554 phalloidin标记（红色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。 | |
Western blot analysis of extracts from 293T cells, untransfected or transfected with 6xHis-Tag fusion protein 1 or 6xHis-Tag fusion protein 2, using His-Tag (D3I1O) XP® Rabbit mAb.Weatern blot分析293T细胞提取物，未转染（-）或转染了6xHis标签融合蛋白1或2，所用抗体为His-Tag (D3I1O) XP® Rabbit mAb。 | |
Immunoprecipitation of His-Tag protein from transfected 293T cells using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or His-Tag (D3I1O) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using His-Tag (D3I1O) XP® Rabbit mAb.免疫沉淀分析293T细胞中His标签蛋白，所用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2) 或 His-Tag (D3I1O) XP® Rabbit mAb (泳道3)。泳道1为10%的细胞提取物。采用His-Tag (D3I1O) XP® Rabbit mAb进行western分析。 | |
Flow cytometric analysis of 293T cells, untransfected (blue) or transfected with a His-myc-Akt plasmid (green), using His-Tag (D3I1O) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.流式细胞术分析293T细胞，未转染（蓝色）或转染了His-myc-Akt质粒（绿色）。使用的抗体是His-Tag (D3I1O) XP® Rabbit mAb。二抗是 Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414。 | |
293T cells were either untransfected (left panel) or transfected with an His-tagged human Stat3 construct (right panel), then treated with Human Interleukin-6 (hIL-6) #8904 (100 ng/ml, 30 min). Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 cells and 5 µl of His-Tag (D3I1O) XP® Rabbit mAb, 5 µl of Stat3α (D1A5) XP® Rabbit mAb #8768, or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Fos Promoter Primers #4663, human IRF-1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.293T细胞未转染（左）或转染了His标签的人Stat3载体（右），再由人Interleukin-6 (hIL-6) #8904处理（100ng/ml,30分钟）。染色质免疫共沉淀使用4 x 106的细胞中的染色质与5 µl的His-Tag (D3I1O) XP® Rabbit mAb，5 µl of Stat3α (D1A5) XP® Rabbit mAb #8768, 或 2 µl of Normal Rabbit IgG #2729，使用的是SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。富集的DNA用实时定量PCR检测，使用SimpleChIP® Human c-Fos Promoter Primers #4663, human IRF-1 promoter primers, 和SimpleChIP® Human α Satellite Repeat Primers #4486。每个样本中免疫沉淀下来的DNA与总染色质的量做对比。 |
