| 货号 | 3469T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | eIF4H (D85F2) XP® Rabbit mAb detects endogenous levels of total eIF4H protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:600) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | A variety of factors contribute to the initiation of translation. Eukaryotic translation initiation factor 4H (eIF4H) was purified to near homogeneity from rabbit reticulocyte lysate and shown to stimulate translation in an assay deficient in eIF4F and eIF4B (1). eIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors eIF4A and eIF4B (1,2). eIF4H was further shown to stimulate the initial rate and extent of eIF4A-mediated mRNA secondary structure unwinding (3). Interaction between eIF4H and the herpes simplex virus shutoff protein (Vhs) appears to be important for Vhs-mediated degradation of mRNA (4). Deletion of a large region of chromosome 7, including the corresponding eIF4H gene, results in Williams-Beuren Syndrome (WBS), an autosomal dominant disorder that can present with cardiovascular problems, mental retardation and distinctive facial features (5). |
| 存放说明 | -20C |
| 计算分子量 | 25, 27 |
| 参考文献 | 1 . Richter-Cook, N.J. et al. (1998) J Biol Chem 273, 7579-87. 2 . Rogers, G.W. et al. (1999) J Biol Chem 274, 12236-44. 3 . Rogers, G.W. et al. (2001) J Biol Chem 276, 30914-22. 4 . Sarma, N. et al. (2008) J Virol 82, 6600-9. 5 . Osborne, L.R. et al. (1996) Genomics 36, 328-36. |
Confocal immunofluorescent analysis of HeLa cells (left) and MCF-7 cells (right) using eIF4H (D85F2) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用eIF4H (D85F2) XP® Rabbit mAb兔单抗 (绿色)，共聚焦免疫荧光观察eIF4H蛋白在HeLa(左图)和MCF-7 (右图)细胞中定位。DY-554 phalloidin (红色)标记微丝蛋白。蓝色伪彩 = DRAQ5® #4084 (DNA 荧光染料)。 | |
Western blot analysis of extracts from HepG2, HeLa and NBT-II cells using eIF4H (D85F2) XP® Rabbit mAb. 使用eIF4H (D85F2) XP® Rabbit mAb兔单抗，免疫印迹（Western Blotting）分析HepG2、 HeLa 和NBT-II细胞系提取物。 |
