货号 | 3535T |
同种亚型 | Rabbit IgG |
反应种属 | Human,Mouse,Rat, |
来源宿主 | Rabbit IgG |
应用 | WB, IP , IHC-P , F , IF-IC |
目标/特异性 | GATA-1 (D52H6) XP® Rabbit mAb detects endogenous levels of GATA-1 protein. |
使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) F (1:400) IF-IC (1:400) |
供应商 | CST |
灵敏度 | Endogenous |
背景 | GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3). |
存放说明 | -20C |
计算分子量 | 43 |
参考文献 | 1 . Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22. 2 . Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010. 3 . Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15. 4 . Pevny, L. et al. (1991) Nature 349, 257-60. 5 . Fujiwara, Y. et al. (1996) Proc Natl Acad Sci USA 93, 12355-8. 6 . Wechsler, J. et al. (2002) Nat Genet 32, 148-52. 7 . Cantor, A.B. (2005) Int J Hematol 81, 378-84. 8 . Mehaffey, M.G. et al. (2001) Blood 98, 2681-8. 9 . Tubman, V.N. et al. (2007) Blood 109, 3297-9. |
Immunohistochemical analysis of paraffin-embedded human bone marrow using GATA-1 (D52H6) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of K-562 (left) and HeLa (right) cells using GATA-1 (D52H6) XP® Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用GATA-1 (D52H6) XP® 兔单抗(绿色)和S6核糖体蛋白(54D2)鼠单抗#2317(红色)对K-562(左)和HeLa(右)细胞进行激光共聚焦荧光分析。蓝色假色=DRAQ® #4084 (DNA荧光染料). | |
Immunohistochemical analysis of formalin-fixed paraffin-embedded undecalcified mouse bone using GATA-1 (D52H6) XP® Rabbit mAb. Note staining of cells in the marrow.使用GATA-1 (D52H6) XP®兔单抗对福尔马林固定石蜡包埋未脱钙处理的小鼠骨骼组织进行免疫组化分析。 | |
Flow cytometric analysis of HeLa cells (red) and K-562 cells (blue) using GATA-1 (D52H6) XP® Rabbit mAb.使用GATA-1 (D52H6) XP® 兔单抗对HeLa细胞(红色)和K-562细胞(蓝色)进行流式细胞分析。 | |
Western blot analysis of lysates from various cell lines using GATA-1 (D52H6) XP® Rabbit mAb.使用GATA-1 (D52H6) XP®兔单抗对多种细胞提取物进行western blot分析。 |