| 货号 | 3544T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IF-IC |
| 目标/特异性 | FABP4 (D25B3) XP® Rabbit mAb detects endogenous levels of total FABP4 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Fatty acid binding proteins (FABPs) bind to fatty acids and other lipids to function as cytoplasmic lipid chaperones (1). They participate in the transport of fatty acids and other lipids to various cellular pathways (2). The predominant fatty acid binding protein found in adipocytes is FABP4, also called adipocyte fatty acid binding protein or aP2. FABP4 is also expressed in macrophages (3). FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes (4). Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE (3). Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders (1). |
| 存放说明 | -20C |
| 计算分子量 | 15 |
| 参考文献 | 1 . Tuncman, G. et al. (2006) Proc Natl Acad Sci U S A 103, 6970-5. 2 . Haunerland, N.H. and Spener, F. (2004) Prog Lipid Res 43, 328-49. 3 . Makowski, L. et al. (2001) Nat. Med. 7, 699-705. 4 . Hotamisligil, G.S. et al. (1996) Science 274, 1377-1379. |
Confocal immunofluorescent analysis of differentiated 3T3-L1 cells (left) and undifferentiated 3T3-L1 cells (right), using FABP4 (D25B3) XP® Rabbit mAb (red). Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光检测分化的3T3-L1细胞（左）和未分化的3T3-L1细胞（右），所用抗体为FABP4 (D25B3) XP@Rabbit mAb兔单抗（红），脂肪滴用 BODIPY 493/503 (绿)染色。蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5 #4084)。 | |
Western blot analysis of extracts from NIH/3T3 and 3T3-L1 cells using FABP4 (D25B3) XP® Rabbit mAb. Wetern blot检测NIH/3T3 和3T3-L1 细胞提取物，所用抗体为FABP4 (D25B3) XP@Rabbit mAb兔单抗。 |
