| 货号 | 3557T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IHC-P , IF-IC |
| 目标/特异性 | TGM2 (D11A6) XP® Rabbit mAb detects endogenous levels of total TGM2 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Transglutaminase 2 (TGM2) is a calcium-dependent enzyme that cross-links both cytosolic and extracellular matrix proteins by catalyzing the formation of bonds between lysine and glutamine residues (1). This bifunctional enzyme also has intrinsic GTPase activity, and it has been suggested that regulation of the transamidase activity might be regulated through a G-protein coupled receptor-signaling pathway (2). In cross-linking peptides, TGM2 helps to regulate cytoskeletal structure, cell migration, apoptosis and cell-matrix adhesion. In addition, the enzyme plays an important role in wound healing and the immune response (3). TGM2 has exhibited kinase activity in vitro, with insulin-like growth factor-binding protein-3 (IGFBP-3) as one possible substrate (4). This widely expressed protein is localized to the cytosol and nucleus, but has also been isolated from the cell surface and extracellular matrix (reviewed in 5). Because of its interaction with a number of different substrates, and its role in the response to injury, TGM2 has been associated with the pathology of a number of human disorders. It has long been recognized as the major autoantigen in celiac disease (6); altered TGM2 expression or activity may be associated with Alzheimer disease, Huntington disease, arteriosclerosis, diabetes, and numerous forms of cancer (reviewed in 7). |
| 存放说明 | -20C |
| 计算分子量 | 78 |
| 参考文献 | 1 . Griffin, M. et al. (2002) Biochem J 368, 377-96. 2 . Jeon, J.H. et al. (2002) Biochem Biophys Res Commun 294, 818-22. 3 . Fesus, L. and Piacentini, M. (2002) Trends Biochem Sci 27, 534-9. 4 . Mishra, S. and Murphy, L.J. (2004) J Biol Chem 279, 23863-8. 5 . Robinson, N.J. et al. (2007) Biol Reprod 77, 648-57. 6 . Dieterich, W. et al. (1997) Nat Med 3, 797-801. 7 . Facchiano, F. et al. (2006) Front Biosci 11, 1758-73. |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using TGM2 (D11A6) XP® Rabbit mAb. 对石蜡包埋的人乳腺使用TGM2 (D11A6) XP® Rabbit mAb进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human placenta using TGM2 (D11A6) XP® Rabbit mAb. 对石蜡包埋的人胎盘使用TGM2 (D11A6) XP® Rabbit mAb进行免疫组化分析。 | |
Western blot analysis of extracts from various cell lines using TGM2 (D11A6) XP® Rabbit mAb. 对多种细胞系使用 TGM2 (D11A6) XP® 兔单克隆抗体进行Western blot分析。 | |
Confocal immunofluorescent analysis of HeLa cells using TGM2 (D11A6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对HeLa细胞使用TGM2 (D11A6) XP® Rabbit mAb（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 |
