| 货号 | 3593T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , IF-IC |
| 目标/特异性 | Grp75 (D13H4) XP® Rabbit mAb detects endogenous levels of total Grp75 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat-inducible (1,2). This protein is essential for transporting many mitochondrial proteins from the cytoplasm to mitochondria (3). Grp75 inactivates the tumor suppressor p53 (4). Studies found that Grp75 is overexpressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (5). Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts (6). |
| 存放说明 | -20C |
| 计算分子量 | 75 |
| 参考文献 | 1 . Kaul, S.C. et al. (1993) Biochem Biophys Res Commun 193, 348-55. 2 . Wadhwa, R. et al. (1993) J Biol Chem 268, 6615-21. 3 . Schneider, H.C. et al. (1994) Nature 371, 768-74. 4 . Wadhwa, R. et al. (1998) J Biol Chem 273, 29586-91. 5 . Wadhwa, R. et al. (2006) Int J Cancer 118, 2973-80. 6 . Kaul, S.C. et al. (2003) Exp Cell Res 286, 96-101. |
Confocal immunofluorescent analysis of MCF-7 cells using Grp75 (D13H4) XP® Rabbit mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对MCF-7细胞使用Grp75 (D13H4) XP® Rabbit mAb（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5®(荧光DNA染料)。 | |
Western blot analysis of extracts from various cell types using Grp75 (D13H4) XP® Rabbit mAb. 对多种细胞系使用Grp75 (D13H4) XP® Rabbit mAb兔单抗进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Grp75 (D13H4) XP® Rabbit mAb in the presence of a control peptide (left) or antigen-sprecific peptide (right). 对石蜡包埋的人肺癌使用Grp75 (D13H4) XP® Rabbit mAb兔单抗进行免疫组织化学分析，其中左图存在对照肽，右图存在抗原特异肽。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Grp75 (D13H4) XP® Rabbit mAb. 对石蜡包埋的人乳腺使用Grp75 (D13H4) XP® Rabbit mAb兔单抗进行免疫组化分析。 |
